AUTHOR=Hunt Benjamin James , Mallon Eamonn B. , Rosato Ezio 

TITLE=In silico Identification of a Molecular Circadian System With Novel Features in the Crustacean Model Organism Parhyale hawaiensis

JOURNAL=Frontiers in Physiology

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2019.01325

DOI=10.3389/fphys.2019.01325

ISSN=1664-042X

ABSTRACT=<p>The amphipod <italic>Parhyale hawaiensis</italic> is a model organism of growing importance in the fields of evolutionary development and regeneration. A small, hardy marine crustacean that breeds year-round with a short generation time, it has simple lab culture requirements and an extensive molecular toolkit including the ability to generate targeted genetic mutant lines. Here we identify canonical core and regulatory clock genes using genomic and transcriptomic resources as a first step in establishing this species as a model in the field of chronobiology. The molecular clock of <italic>P. hawaiensis</italic> lacks orthologs of the canonical circadian genes <italic>cryptochrome 1</italic> and <italic>timeless</italic>, in common with the mammalian system but in contrast to many arthropods including <italic>Drosophila melanogaster</italic>. Furthermore the predicted CLOCK peptide is atypical and CRY2 shows an extended 5′ region of unknown function. These results appear to be shared by two other amphipod species.</p>