AUTHOR=Martinez-Mateu Laura , Saiz Javier , Aromolaran Ademuyiwa S. 

TITLE=Differential Modulation of IK and ICa,L Channels in High-Fat Diet-Induced Obese Guinea Pig Atria

JOURNAL=Frontiers in Physiology

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2019.01212

DOI=10.3389/fphys.2019.01212

ISSN=1664-042X

ABSTRACT=<p>Obesity mechanisms that make atrial tissue vulnerable to arrhythmia are poorly understood. Voltage-dependent potassium (<italic>I<sub>K</sub></italic>, <italic>I<sub>Kur</sub></italic>, and <italic>I<sub>K1</sub></italic>) and L-type calcium currents (<italic>I<sub>Ca,L</sub></italic>) are electrically relevant and represent key substrates for modulation in obesity. We investigated whether electrical remodeling produced by high-fat diet (HFD) alone or in concert with acute atrial stimulation were different. Electrophysiology was used to assess atrial electrical function after short-term HFD-feeding in guinea pigs. HFD atria displayed spontaneous beats, increased <italic>I<sub>K</sub></italic> (<italic>I<sub>Kr</sub> + I<sub>Ks</sub></italic>) and decreased <italic>I<sub>Ca,L</sub></italic> densities. Only with pacing did a reduction in <italic>I<sub>Kur</sub></italic> and increased <italic>I<sub>K1</sub></italic> phenotype emerge, leading to a further shortening of action potential duration. Computer modeling studies further indicate that the measured changes in potassium and calcium current densities contribute prominently to shortened atrial action potential duration in human heart. Our data are the first to show that multiple mechanisms (shortened action potential duration, early afterdepolarizations and increased incidence of spontaneous beats) may underlie initiation of supraventricular arrhythmias in obese guinea pig hearts. These results offer different mechanistic insights with implications for obese patients harboring supraventricular arrhythmias.</p>