AUTHOR=Tang Guang-Hui , Xiong Ying , Liu Yi , Song Zhong-Hao , Yang Yang , Shen Guang-Mao , Wang Jin-Jun , Jiang Hong-Bo 

TITLE=The Transcription Factor MafB Regulates the Susceptibility of Bactrocera dorsalis to Abamectin via GSTz2

JOURNAL=Frontiers in Physiology

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2019.01068

DOI=10.3389/fphys.2019.01068

ISSN=1664-042X

ABSTRACT=<p>Pesticide resistance is a serious problem that poses a major challenge to pest control. One of the most potent resistance mechanisms is the overexpression of genes coding for detoxification enzymes. The expression of detoxification genes is regulated by a series of transcription factors. Previous studies have revealed that the increased expression of detoxification genes contributes to the insecticide tolerance of <italic>Bactrocera dorsalis.</italic> Our objective was thus to identify the transcription factors involved in this process. Temporal expression profiles showed that the transcription factor <italic>MafB</italic> and detoxification genes were expressed highly in the fat body. Further analysis showed that the expression of <italic>MafB</italic>, <italic>GSTz2</italic>, and <italic>CYP473A3</italic> was induced by abamectin. Disruption of the <italic>MafB</italic> transcription factor through RNA interference decreased the transcript levels of <italic>GSTz2</italic> and <italic>CYP473A3</italic> and increased the susceptibility to abamectin significantly. Direct silencing of the expression of <italic>GSTz2</italic> also increased susceptibility to abamectin, while <italic>CYP473A3</italic> did not. In conclusion, these results suggest that the expression of <italic>GSTz2</italic> and <italic>CYP473A3</italic> was regulated by the transcription factor <italic>MafB</italic>, and the up-regulation of <italic>GSTz2</italic> via <italic>MafB</italic> decreased the susceptibility of <italic>B. dorsalis</italic> to abamectin.</p>