AUTHOR=Zafarnia Sara , Mrugalla Anna , Rix Anne , Doleschel Dennis , Gremse Felix , Wolf Stephanie D. , Buyel Johannes F. , Albrecht Ute , Bode Johannes G. , Kiessling Fabian , Lederle Wiltrud TITLE=Non-invasive Imaging and Modeling of Liver Regeneration After Partial Hepatectomy JOURNAL=Frontiers in Physiology VOLUME=10 YEAR=2019 URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2019.00904 DOI=10.3389/fphys.2019.00904 ISSN=1664-042X ABSTRACT=
The liver has a unique regenerative capability upon injury or partial resection. The regeneration process comprises a complex interplay between parenchymal and non-parenchymal cells and is tightly regulated at different scales. Thus, we investigated liver regeneration using multi-scale methods by combining non-invasive imaging with immunohistochemical analyses. In this context, non-invasive imaging can provide quantitative data of processes involved in liver regeneration at organ and body scale. We quantitatively measured liver volume recovery after 70% partial hepatectomy (PHx) by micro computed tomography (μCT) and investigated changes in the density of CD68+ macrophages by fluorescence-mediated tomography (FMT) combined with μCT using a newly developed near-infrared fluorescent probe. In addition, angiogenesis and tissue-resident macrophages were analyzed by immunohistochemistry. Based on the results, a model describing liver regeneration and the interactions between different cell types was established.