AUTHOR=Kanakala Surapathrudu , Kontsedalov Svetlana , Lebedev Galina , Ghanim Murad 

TITLE=Plant-Mediated Silencing of the Whitefly Bemisia tabaci Cyclophilin B and Heat Shock Protein 70 Impairs Insect Development and Virus Transmission

JOURNAL=Frontiers in Physiology

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2019.00557

DOI=10.3389/fphys.2019.00557

ISSN=1664-042X

ABSTRACT=<p>The whitefly <italic>B. tabaci</italic> is a global pest and transmits extremely important plant viruses especially begomoviruses, that cause substantial crop losses. <italic>B. tabaci</italic> is one of the top invasive species worldwide and have developed resistance to all major pesticide classes. One of the promising alternative ways for controlling this pest is studying its genetic makeup for identifying specific target proteins which are critical for its development and ability to transmit viruses. <italic>Tomato yellow leaf curl virus</italic> (TYLCV) is the most economically important and well-studied begomovirus transmitted by <italic>B. tabaci</italic>, in a persistent-circulative manner. Recently, we reported that <italic>B. tabaci</italic> Cyclophilin B (<italic>CypB</italic>) and heat shock protein 70 proteins (<italic>hsp70</italic>) interact and co-localize with TYLCV in the whitefly midgut, on the virus transmission pathway, and that both proteins have a significant role in virus transmission. Here, we extended the previous work and used the <italic>Tobacco rattle virus</italic> (TRV) plant-mediated RNA silencing system for knocking down both genes and testing the effect of their silencing on whitefly viability and virus transmission. Portions of these two genes were cloned into TRV constructs and tomato plants were infected and used for whitefly feeding and transmission experiments. Following whitefly feeding on TRV-plants, the expression levels of <italic>cypB</italic> and <italic>hsp70</italic> in adult <italic>B. tabaci</italic> significantly decreased over 72 h feeding period. The knockdown in the expression of both genes was further shown in the first generation of silenced whiteflies, where phenotypic abnormalities in the adult, wing, nymph and bacteriosomes development and structure were observed. Additionally, high mortality rates that reached more than 80% among nymphs and adults were obtained. Finally, silenced whitefly adults with both genes showed decreased ability to transmit TYLCV under lab conditions. Our results suggest that plant-mediated silencing of both <italic>cypB</italic> and <italic>hsp70</italic> have profound effects on whitefly development and its ability to transmit TYLCV.</p>