AUTHOR=Qiu Jian-Feng , Li Xue , Cui Wen-Zhao , Liu Xiao-Fei , Tao Hui , Yang Kun , Dai Tai-Ming , Sima Yang-Hu , Xu Shi-Qing 

TITLE=Inhibition of Period Gene Expression Causes Repression of Cell Cycle Progression and Cell Growth in the Bombyx mori Cells

JOURNAL=Frontiers in Physiology

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2019.00537

DOI=10.3389/fphys.2019.00537

ISSN=1664-042X

ABSTRACT=<p>Circadian clock system disorders can lead to uncontrolled cell proliferation, but the molecular mechanism remains unknown. We used a <italic>Bombyx mori</italic> animal model of single <italic>Period</italic> gene (<italic>BmPer</italic>) expression to investigate this mechanism. A slow growing developmental cell model (Per-KD) was isolated from a <italic>B. mori</italic> ovarian cell line (BmN) by continuous knock down of <italic>BmPer</italic> expression. The effects of <italic>BmPer</italic> expression knockdown (Per-KD) on cell proliferation and apoptosis were opposite to those of <italic>m/hPer1</italic> and <italic>m/hPer2</italic> in mammals. The knockdown of <italic>BmPer</italic> expression led to cell cycle deceleration with shrinking of the BmN cell nucleus, and significant inhibition of nuclear DNA synthesis and cell proliferation. It also promoted autophagy via the lysosomal pathway, and accelerated apoptosis via the caspase pathway.</p>