AUTHOR=Zhou Cao , Yang Hong , Wang Zhao , Long Gui-Yun , Jin Dao-Chao 

TITLE=Protective and Detoxifying Enzyme Activity and ABCG Subfamily Gene Expression in Sogatella furcifera Under Insecticide Stress

JOURNAL=Frontiers in Physiology

VOLUME=9

YEAR=2019

URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2018.01890

DOI=10.3389/fphys.2018.01890

ISSN=1664-042X

ABSTRACT=<p><italic>Sogatella furcifera</italic>, an important migratory pest of rice, has substantial detrimental effects on rice production. To clarify the mechanism whereby <italic>S. furcifera</italic> responds to insecticide stress, we measured the activity of its protective [superoxide dismutase (SOD); peroxidase (POD); catalase (CAT)] and detoxifying [carboxylesterase (CarE); glutathione <italic>S</italic>-transferase (GST); mixed-function oxidase (MFO)] enzymes and the expression levels of its ATP-binding cassette subfamily G (<italic>ABCG</italic>) transporter genes in response to sublethal concentrations (LC<sub>10</sub> and LC<sub>25</sub>) of the insecticides thiamethoxam, buprofezin, and abamectin. On the bases of the transcriptome data and the <italic>ABCG</italic> genes of <italic>Laodelphax striatellus</italic>, we obtained 14 full-length <italic>ABCG</italic> sequences for <italic>S. furcifera</italic>. RT-qPCR results showed that 13, 12, and 9 <italic>sfABCG</italic> genes were upregulated in the presence of thiamethoxam, buprofezin, and abamectin, respectively, at LC<sub>10</sub>. Moreover, 13 and 7 <italic>sfABCG</italic> genes were upregulated following treatment with thiamethoxam and abamectin, respectively, at LC<sub>25</sub>. Enzyme activity assays showed that although thiamethoxam, buprofezin, and abamectin induced GST, CarE, CAT, POD, and SOD activity, they did so at different concentrations and exposure times. The activity of MFO was generally inhibited with prolonged exposure to the three insecticides, with the inhibitory effect being most significant at 72 h. These results indicate that <italic>S. furcifera</italic> differs in its response to different types or concentrations of insecticides. Taken together, our results lay the foundations for gaining a deeper understanding of the mechanisms underlying the adaptation of <italic>S. furcifera</italic> to different types of insecticides, which would be of considerable significance for the development of effective pest management strategies.</p>