AUTHOR=Yin Zhen-Juan , Dong Xiao-Lin , Kang Kui , Chen Hao , Dai Xiao-Yan , Wu Guang-An , Zheng Li , Yu Yi , Zhai Yi-Fan 

TITLE=FoxO Transcription Factor Regulate Hormone Mediated Signaling on Nymphal Diapause

JOURNAL=Frontiers in Physiology

VOLUME=9

YEAR=2018

URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2018.01654

DOI=10.3389/fphys.2018.01654

ISSN=1664-042X

ABSTRACT=<p>Diapause is a complex physiological adaptation phenotype, and the transcription factor Forkhead-box O (FoxO) is a prime candidate for activating many of its diverse regulatory signaling pathways. Hormone signaling regulates nymphal diapause in <italic>Laodelphax striatellus</italic>. Here, the function of the <italic>FoxO</italic> gene isolated from <italic>L. striatellus</italic> was investigated. After knocking-down <italic>LsFoxO</italic> in diapausal nymphs using RNA interference, the titers of juvenile hormone III and some cold-tolerance substances decreased significantly, and the duration of the nymphal developmental period was severely shorted to 25.5 days at 20°C under short day-length (10 L:14 D). To determine how <italic>LsFoxO</italic> affects nymphal diapause, analyses of RNA-sequencing transcriptome data after treatment with <italic>LsFoxO</italic>–RNA interference was performed. The differentially expressed genes affected carbohydrate, amino acid and fatty acid metabolism, and phosphatidylinositol 3-kinase/protein kinase B signaling pathway. Thus, <italic>LsFoxO</italic> acts on <italic>L. striatellus</italic> nymphal diapause and is, therefore, a potential target gene for pest control. This study may lead to new information on the regulation of nymphal diapause in this important pest.</p>