AUTHOR=Li Zhao-Qun , Cai Xiao-Ming , Luo Zong-Xiu , Bian Lei , Xin Zhao-Jun , Chu Bo , Liu Yan , Chen Zong-Mao 

TITLE=Comparison of Olfactory Genes in Two Ectropis Species: Emphasis on Candidates Involved in the Detection of Type-II Sex Pheromones

JOURNAL=Frontiers in Physiology

VOLUME=9

YEAR=2018

URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2018.01602

DOI=10.3389/fphys.2018.01602

ISSN=1664-042X

ABSTRACT=<p>The sibling species <italic>Ectropis grisescens</italic> and <italic>E. obliqua</italic> are the major chewing tea pests in China. A difference in sex pheromone components plays a central role in premating isolation in these two species. To investigate the mechanism of premating isolation in these two <italic>Ectropis</italic> species, we sequenced the transcriptomes of the antennae of female and male <italic>E. obliqua</italic> individuals and performed phylogenetic analyses, abundance analyses, and tissue expression profile analyses to compare the olfactory genes involved in the detection of sex pheromones. A total of 36 odorant-binding proteins (OBPs) and 52 olfactory receptors (ORs) were identified in <italic>E. obliqua</italic>. Phylogenetic analyses showed that EoblOBP2, 3, and 25 were grouped in the pheromone-binding protein clade with EgriOBP2, 3, 25, and another lepidopteran PBP. EoblOR25 and 28 were grouped with EgriOR25, 28, and pheromone receptors for the detection of Type-I sex pheromone components. EoblOR24, 31, 37, and 44 were grouped with EgriOR24, 31, 37, and 44. All of these 4 EoblORs and 4 <italic>EgriORs</italic> showed higher abundance in male antennae than in female ones. Therefore, OBP2, 3, 25 and OR24, 31, 37, 44 of <italic>E. grisescens</italic> and <italic>E. obliqua</italic> might be responsible for sex pheromone component detection. However, the sequences of these genes in <italic>E. grisescens</italic> and <italic>E. obliqua</italic> were more than 90% identical. This indicates that these orthologous genes might play similar roles in the detection of sex pheromones. In contrast, the observed <italic>OBPs</italic> and <italic>ORs</italic> differed in abundance between the antennae of the two <italic>Ectropis</italic> species. Therefore, we speculate that these two <italic>Ectropis</italic> species use the different transcript levels of PRs to differentiate sex pheromone components. The results of the present study might contribute in deciphering the mechanism for premating isolation in these species and may be of use in devising strategies for their management.</p>