AUTHOR=Yang Shui-Bo , Tan Xiao-Ying , Zhang Dian-Guang , Cheng Jie , Luo Zhi 

TITLE=Identification of 10 SUMOylation-Related Genes From Yellow Catfish Pelteobagrus fulvidraco, and Their Transcriptional Responses to Carbohydrate Addition in vivo and in vitro

JOURNAL=Frontiers in Physiology

VOLUME=9

YEAR=2018

URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2018.01544

DOI=10.3389/fphys.2018.01544

ISSN=1664-042X

ABSTRACT=<p>SUMOylation is a kind of important post-translational modification. In the present study, we identified 10 key genes involved in SUMOylation and deSUMOylation (<italic>sumo1, sumo2, sumo3, sae1, uba2, ubc9, pias1, senp1, senp2</italic>, and <italic>senp3</italic>) in yellow catfish <italic>Pelteobagrus fulvidraco</italic>, investigated their tissue expression patterns and transcriptional responses to carbohydrate addition both <italic>in vivo</italic> and <italic>in vitro</italic>. All of these members shared similar domains to their orthologous genes of other vertebrates. Their mRNAs were widely expressed in all the tested tissues, but at variable levels. Dietary carbohydrate levels differentially influenced the mRNA levels of these genes in liver, muscle, testis, and ovary of yellow catfish. Their mRNA levels in primary hepatocytes were differentially responsive to glucose addition. Our study would contribute to our understanding into the molecular basis of SUMOylation modification and into the potential SUMOylation function in the carbohydrate utilization in fish.</p>