AUTHOR=Chi Jing , Li Zizhuo , Hong Xiaojian , Zhao Tong , Bie Yueyue , Zhang Wen , Yang Jiaxing , Feng Ziming , Yu Zhouqi , Xu Qiannan , Zhao Luqi , Liu Weifan , Gao Yunan , Yang Hongxiao , Yang Jiemei , Liu Jiaren , Yang Wei 

TITLE=Inhalation of Hydrogen Attenuates Progression of Chronic Heart Failure via Suppression of Oxidative Stress and P53 Related to Apoptosis Pathway in Rats

JOURNAL=Frontiers in Physiology

VOLUME=9

YEAR=2018

URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2018.01026

DOI=10.3389/fphys.2018.01026

ISSN=1664-042X

ABSTRACT=<p><bold>Background:</bold> Continuous damage from oxidative stress and apoptosis are the important mechanisms that facilitate chronic heart failure (CHF). Molecular hydrogen (H<sub>2</sub>) has potentiality in the aspects of anti-oxidation. The objectives of this study were to investigate the possible mechanism of H<sub>2</sub> inhalation in delaying the progress of CHF.</p><p><bold>Methods and Results:</bold> A total of 60 Sprague-Dawley (SD) rats were randomly divided into four groups: Sham, Sham treated with H<sub>2</sub>, CHF and CHF treated with H<sub>2</sub>. Rats from CHF and CHF treated with H<sub>2</sub> groups were injected isoprenaline subcutaneously to establish the rat CHF model. One month later, the rat with CHF was identified by the echocardiography. After inhalation of H<sub>2</sub>, cardiac function was improved vs. CHF (<italic>p</italic> &lt; 0.05), whereas oxidative stress damage and apoptosis were significantly attenuated (<italic>p</italic> &lt; 0.05). In this study, the mild oxidative stress was induced in primary cardiomyocytes of rats, and H<sub>2</sub> treatments significantly reduced oxidative stress damage and apoptosis in cardiomyocytes (<italic>p</italic> &lt; 0.05 or <italic>p</italic> &lt; 0.01). Finally, as a pivotal transcription factor in reactive oxygen species (ROS)-apoptosis signaling pathway, the expression and phosphorylation of p53 were significantly reduced by H<sub>2</sub> treatment in this rat model and H9c2 cells (<italic>p</italic> &lt; 0.05 or <italic>p</italic> &lt; 0.01).</p><p><bold>Conclusion:</bold> As a safe antioxidant, molecular hydrogen mitigates the progression of CHF via inhibiting apoptosis modulated by p53. Therefore, from the translational point of view and speculation, H<sub>2</sub> is equipped with potential therapeutic application as a novel antioxidant in protecting CHF in the future.</p>