AUTHOR=Zhang Jin , Ma Wenge , He Yan , Dawar Farman U. , Xiong Shuting , Mei Jie 

TITLE=Potential Contributions of miR-200a/-200b and Their Target Gene–Leptin to the Sexual Size Dimorphism in Yellow Catfish

JOURNAL=Frontiers in Physiology

VOLUME=8

YEAR=2017

URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2017.00970

DOI=10.3389/fphys.2017.00970

ISSN=1664-042X

ABSTRACT=<p>Sexual size dimorphism is the consequence of differential expression of sex-biased genes related to feeding and growth. Leptin is known to regulate energy balance by regulating food intake. In order to investigate the molecular mechanism of sexual size dimorphism in yellow catfish (<italic>Pelteobagrus fulvidraco</italic>), the expression of <italic>leptin</italic> (<italic>lep</italic>) and its functional receptor (<italic>lepr</italic>) were detected during larval development. Both <italic>lep</italic> and <italic>lepr</italic> have lower expression in males than in females during 1–4 weeks post hatching. 17a-Methyltestosterone (MT) treatment resulted in decreased expression of <italic>lep</italic> and <italic>lepr</italic> in both male and female larval fish. Interestingly, the mRNA levels of <italic>lep</italic> and <italic>lepr</italic> in juvenile male were significantly decreased compared with juvenile female during short-term fasting periods. <italic>Lep</italic> was predicted to be a potential target of miR-200a and miR-200b that had an opposite expression pattern to <italic>lep</italic> in male and female larvas. The results of luciferase reporter assay suggested that <italic>lep</italic> is a target of miR-200a/-200b. Subsequently, male hormone and fasting treatment have opposite effects on the expression of miR-200a/-200b and <italic>lep</italic> between males and females. In summary, our results suggest that sexual size dimorphism in fish species is probably caused by the sexually dimorphic expression of <italic>leptin</italic>, which could be negatively regulated by miR-200a/-200b.</p>