AUTHOR=Natsiou Despoina , Granchi Zoraide , Mitsiadis Thimios A. , Jimenez-Rojo Lucia 

TITLE=Generation of Spheres from Dental Epithelial Stem Cells

JOURNAL=Frontiers in Physiology

VOLUME=8

YEAR=2017

URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2017.00007

DOI=10.3389/fphys.2017.00007

ISSN=1664-042X

ABSTRACT=<p>The <italic>in vitro</italic> three-dimensional sphere model has already been established as an important tool in fundamental sciences. This model facilitates the study of a variety of biological processes including stem cell/niche functions and tissue responses to injury and drugs. Here we describe the complete protocol for the <italic>in vitro</italic> formation of spheres originated from the epithelium of rodent incisors. In addition, we show that in these spheres cell proliferation is maintained, as well as the expression of several key molecules characterizing stem cells such as Sox2 and p63. These epithelial dentospheres could be used as an <italic>in vitro</italic> model system for stem cell research purposes.</p>