AUTHOR=Greer-Short Amara , Poelzing Steven 

TITLE=Distinguishing between overdrive excited and suppressed ventricular beats in guinea pig ventricular myocardium

JOURNAL=Frontiers in Physiology

VOLUME=6

YEAR=2015

URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2015.00014

DOI=10.3389/fphys.2015.00014

ISSN=1664-042X

ABSTRACT=<p>Rapid ventricular pacing rates induces two types of beats following pacing cessation: recovery cycle length (RCL) prolongation (overdrive suppression) and RCL shortening (overdrive excitation). The goals of this study were to compare common experimental protocols for studying triggered activity in whole-heart preparations and differentiate between recovery beats using a new methodology. Post-pacing recovery beat cycle length (RCL) and QRS were normalized to pre-paced R-R and QRS intervals and analyzed using a K-means clustering algorithm. Control hearts only produced suppressed beats: RCL ratio increased with rapid pacing (25 ± 4.0%, <italic>n</italic> = 10) without changing QRS duration. Rapid pacing during hypercalcemia + hypothermia (5.5 mM and 34°C) produced significantly earlier excited beats (53 ± 14%, <italic>n</italic> = 5) with wider QRS durations (58 ± 6.3%, <italic>n</italic> = 5) than suppressed beats. Digoxin + hypothermia (0.75 μM) produced the most excited beats with significantly earlier RCL (44 ± 3.2%, <italic>n</italic> = 6) and wider QRS (60 ± 3.1%, <italic>n</italic> = 6) ratios relative to suppressed beats. Increasing pacing further shortened RCL (30 ± 7.8%, <italic>n</italic> = 6). In a prospective study, TTX (100 nM) increased RCL ratio (15 ± 6.0%, <italic>n</italic> = 10) without changing the QRS duration of excited beats. The algorithm was compared to a cross-correlation analysis with 93% sensitivity and 94% specificity. This ECG based algorithm distinguishes between triggered and automatic activity.</p>