AUTHOR=Stride Nis , Larsen Steen , Treebak Jonas T., Hansen Christina N., Hey-Mogensen Martin , Speerschneider Tobias , Jensen Thomas E., Jeppesen Jacob , Wojtaszewski Jørgen F., Richter Erik A., Køber Lars , Dela Flemming TITLE=5′-AMP Activated Protein Kinase is Involved in the Regulation of Myocardial β-Oxidative Capacity in Mice JOURNAL=Frontiers in Physiology VOLUME=3 YEAR=2012 URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2012.00033 DOI=10.3389/fphys.2012.00033 ISSN=1664-042X ABSTRACT=
5′-adenosine monophosphate-activated protein kinase (AMPK) is considered central in regulation of energy status and substrate utilization within cells. In heart failure the energetic state is compromised and substrate metabolism is altered. We hypothesized that this could be linked to changes in AMPK activity and we therefore investigated mitochondrial oxidative phosphorylation capacity from the oxidation of long- and medium-chain fatty acids (LCFA and MCFA) in cardiomyocytes from young and old mice expressing a dominant negative AMPKα2 (AMPKα2-KD) construct and their wildtype (WT) littermates. We found a 35–45% (