AUTHOR=Rotman-Nativ Noa , Shaked Natan T. TITLE=Live Cancer Cell Classification Based on Quantitative Phase Spatial Fluctuations and Deep Learning With a Small Training Set JOURNAL=Frontiers in Physics VOLUME=9 YEAR=2021 URL=https://www.frontiersin.org/journals/physics/articles/10.3389/fphy.2021.754897 DOI=10.3389/fphy.2021.754897 ISSN=2296-424X ABSTRACT=

We present an analysis method that can automatically classify live cancer cells from cell lines based on a small data set of quantitative phase imaging data without cell staining. The method includes spatial image analysis to extract the cell phase spatial fluctuation map, derived from the quantitative phase map of the cell measured without cell labeling, thus without prior knowledge on the biomarker. The spatial fluctuations are indicative of the cell stiffness, where cancer cells change their stiffness as cancer progresses. In this paper, the quantitative phase spatial fluctuations are used as the basis for a deep-learning classifier for evaluating the cell metastatic potential. The spatial fluctuation analysis performed on the quantitative phase profiles before inputting them to the neural network was proven to increase the classification results in comparison to inputting the quantitative phase profiles directly, as done so far. We classified between primary and metastatic cancer cells and obtained 92.5% accuracy, in spite of using a small training set, demonstrating the method potential for objective automatic clinical diagnosis of cancer cells in vitro.