AUTHOR=Liang Yong , Chen Xiaohu , Sun Zhenglong , Wen Gang , Chen Chong , Wang Libo , Jin Xin , Zhang Jie , Yang Guang , Gao Jing , Li Hui , Li Simin TITLE=High Dynamic Range Structured Illumination Microscope Based on Multiple Exposures JOURNAL=Frontiers in Physics VOLUME=9 YEAR=2021 URL=https://www.frontiersin.org/journals/physics/articles/10.3389/fphy.2021.648174 DOI=10.3389/fphy.2021.648174 ISSN=2296-424X ABSTRACT=

Structured illumination microscope (SIM) can double the spatial resolution by using fringed pattern illumination. However, for samples with large intra-scene dynamic ranges, such as clustered objects, SIM fails to reconstruct high-quality images and often exhibits strong artifacts. Herein, we present a high dynamic range SIM (HDR-SIM) method using a multi-exposure acquisition strategy. With HDR-SIM, individual and clustered microspheres and vesicles with 420 times intensity differences can be visualized in the same scene while the delicate structures of the sample were preserved effectively.