AUTHOR=Gallian Brandon , Zaeimian Masoumeh Saber , Hau Derrick , AuCoin David , Zhu Xiaoshan 

TITLE=A Highly Sensitive Time-Gated Fluorescence Immunoassay Platform Using Mn-Doped AgZnInS/ZnS Nanocrystals as Signal Transducers

JOURNAL=Frontiers in Physics

VOLUME=8

YEAR=2021

URL=https://www.frontiersin.org/journals/physics/articles/10.3389/fphy.2020.625424

DOI=10.3389/fphy.2020.625424

ISSN=2296-424X

ABSTRACT=<p>In this work, a time-gated immunoassay platform using low-energy excitable and fluorescence long-lived Mn:AgZnInS/ZnS nanocrystals as signal transducers was developed and applied to the detection of the capsular polysaccharide (CPS) of <italic>Burkholderia pseudomallei</italic>, a Gram-negative bacterium that is the causative agent of melioidosis. CPS is a high molecular weight antigen displayed and is shed from the outer membrane of <italic>B. pseudomallei</italic>. The immunoassay using the time-gated platform presents a limit of detection at around 23 pg/ml when CPS is spiked in human serum.</p>