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ORIGINAL RESEARCH article

Front. Photobiol.
Sec. Photoecology and Environmental Photobiology
Volume 2 - 2024 | doi: 10.3389/fphbi.2024.1441713
This article is part of the Research Topic Aquatic Phototrophic Communities: Insights into Light-driven Ecological Dynamics View all articles

Non-enzymatic oxylipin production in a mudflat microphytobenthic biofilm: evidence of a diatom response to light

Provisionally accepted
Caroline Doose Caroline Doose 1*Camille Oger Camille Oger 2Lindsay Mas-Normand Lindsay Mas-Normand 2Thierry Durand Thierry Durand 2Cédric Hubas Cédric Hubas 1
  • 1 Muséum National d'Histoire Naturelle, Paris, France
  • 2 UMR5247 Institut des Biomolécules Max Mousseron (IBMM), Montpellier, Languedoc-Roussillon, France

The final, formatted version of the article will be published soon.

    Microphytobenthos (MPB) is a diatom dominated microbial community of primary producers inhabiting the mudflat sediments. On one hand, the benthic diatoms display photo-protective strategies to face extreme light variations susceptible to generate cellular oxidative stress. On the other hand, oxidative stress induces the production of reactive oxygen species (ROS) that generate oxylipins, oxygenated metabolites of polyunsaturated fatty acids (PUFAs), which are among the known chemical mediators in diatoms. However, non-enzymatically generated oxylipins known as isoprostanoids or isofuranoids are poorly studied in diatoms. To better understand the roles of the latter in migrational MPB light response, we investigated the effect of different irradiances corresponding to dark (D), low light (LL, 50 and 100 µmol. photons. m−2. s−1PAR), medium light (ML, 250 µmol. photons. m−2. s−1 PAR), high light (HL, 500, 750 and 1000 µmol. photons. m−2. s−1PAR), on the isoprostanoids production by the biofilm’s organisms. The PUFAs precursors of the varying oxylipins evidenced a diatoms response to irradiance. Under 1000 PAR condition, the total amount of isoprotanoids increased, indicating an oxidative stress response. Isoprostanes (IsoPs) and prostaglandins (PGs) characterized the HL conditions and evidenced lipid peroxidation probably linked to the higher generation of ROS by the photosynthesis. On the contrary, the phytoprostanes (PhytoPs) characterized the LL and ML, where the ROS scavengers were probably not overwhelmed. This first investigation of non-enzymatic oxylipin production by a microphytobenthic biofilm under different irradiances highlighted the interest to explore their potential signaling roles related to MPB light responses.

    Keywords: microphytobenthos, Oxylipins, isprostanoids, light acclimations, Mudflat Biofilm

    Received: 31 May 2024; Accepted: 16 Aug 2024.

    Copyright: © 2024 Doose, Oger, Mas-Normand, Durand and Hubas. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Caroline Doose, Muséum National d'Histoire Naturelle, Paris, 75005, France

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