ORIGINAL RESEARCH article
Front. Pharmacol.
Sec. Ethnopharmacology
Volume 16 - 2025 | doi: 10.3389/fphar.2025.1569052
Menthacarin, a proprietary combination of peppermint and caraway oil alters cultured human fecal microbiota composition resulting in increased SCFA production
Provisionally accepted
- 1Dr. Willmar Schwabe GmbH & Co. KG, Karlsruhe, Germany
- 2Acaryon GmbH, Berlin, Germany
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Background: Disruptions in gut microbiota metabolism may contribute to the pathophysiology of disorders of gut-brain interaction and correction of intestinal dysbiosis is considered a promising therapeutic approach. Menthacarin, a proprietary fixed combination of Mentha x piperita L. and Carum carvi L. essential oils, is used clinically for the treatment of functional dyspepsia and irritable bowel syndrome. Rodent model data indicate that treatment effects on visceral hypersensitivity by Menthacarin could be mediated via normalization of gut dysbiosis. However, the impact of Menthacarin on human bacterial gut microbiota has not yet been studied.The aim of the present study was to assess whether Menthacarin affects the composition and metabolic activity of human fecal microbiota.Methods: Fecal slurry samples from 10 healthy volunteers were cultivated for 36 hours under anoxic conditions with and without Menthacarin. Relative bacterial abundance at the phylum and genus levels was evaluated using 16S rRNA metagenomic analysis. Short chain fatty acids (SCFA) in the supernatants were measured using LC-MS technology.Results: Menthacarin induced robust changes in microbial composition at both the phylum and genus levels among the ten donor microbiomes. The relative abundance of Firmicutes (+13.6 ± 8.6%) and Actinobacteria (+54.9 ± 47.6% ) significantly increased, while Bacteroidetes (-27.7 ± 21.9%) and Proteobacteria (-25.7 ± 12.3%) significantly decreased in the presence of Menthacarin. At the genus level, the most notable changes were significant increases in Bifidobacterium (+105.1 ± 78.4%) and several SCFA producing genera accompanied by a significant decrease in genera containing members involved in pro-inflammatory processes. In addition, Menthacarin significantly increased the levels of several SCFA, namely propionate, butyrate, isobutyrate, valerate, and isovalerate.Menthacarin alters microbiota composition and enhances SCFA production in human microbiota samples under in vitro conditions. These effects may contribute to the clinical benefits observed with Menthacarin treatment.
Keywords: microbiota, Menthacarin, peppermint, Caraway, short-chain fatty acids, functional dyspepsia, Irritable Bowel Syndrome
Received: 31 Jan 2025; Accepted: 24 Mar 2025.
Copyright: © 2025 Lehner, Ulsemer and Christochowitz. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Martin D. Lehner, Dr. Willmar Schwabe GmbH & Co. KG, Karlsruhe, Germany
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