A double-layer PLGA/CoI-MeHA tissue engineering scaffold for urethral reconstruction

Mingyang Chang ¹ Tan Qin Yuan ² Ge Bian ¹ Zhang Ming ¹ Jianing Lv ¹ Junjie Su ¹ Xiaoqing Wang ^1*

• ¹ The First Hospital of Jilin University, Changchun, China
• ² Department of Urolory, The People's Hospital of Jimo.Qingdao, Qingdao, China

Urethral injury caused by various reasons usually leads to urethral stricture. And severe urethral stricture can further induce complications such as bladder stones, fistulas, sepsis, and even renal failure. At present, surgical methods such as urethral reconstruction and end-to-end anastomosis are commonly used to solve this problem. But this treatment method often has a high recurrence rate. So simply relying on the repair of surrounding autologous tissue cells to reconstruct the urethra is difficult to achieve long-term stability, and constructing a suitable urethral graft is an effective and feasible solution. Therefore, we designed and prepared a double-layer polylactic-co-glycolic acid /collagen type I-methacrylated hyaluronic acid tissue engineering scaffold to better simulate the natural anatomy of the urethra and achieve urethral tissue regeneration and reconstruction in patients with urethral stricture and Hypospadias caused by various reasons. The double-layer tissue engineering scaffold was generated using electrospinning and light curing technology which displayed sufficient mechanical strength, good biocompatibility, degradation characteristics, and effectively simulated the natural anatomy of urethra, achieving satisfactory urethral defect reconstruction results.