Chromium and Formoterol Therapy for obesity-induced Asthma in Rats

Rania T Ibrahim ¹ Yasser M Moustafa ² Maha Abdullah Alwaili ³ Amjad N Alrebdi ³ Afaf Alharthi ⁴ Noha R Noufal ⁵ Dina Khodeer ^2*

• ¹ Egypt Healthcare Authority, Ismailia, Egypt
• ² Faculty of Pharmacy, Suez Canal University, Ismaïlia, Egypt
• ³ Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia
• ⁴ Taif University, Ta'if, Saudi Arabia
• ⁵ Dar Al Uloom University, Riyadh, Saudi Arabia

The development of asthma is impacted by fat. Asthma is more common in obese persons. The purpose of the experimental study is to determine how chromium, formoterol, and their combination can improve the quality of life for obese people with lung anomalies.Thirty-six male Wistar rats were divided into six groups: control (C), obesity (CO), obeseasthma (COA), and obese-asthma groups treated with formoterol (OAF), chromium (OACR), or both (OACRF). Except for group C, all groups received a high-fat diet for four weeks. Subsequently, ovalbumin (OVA) was administered subcutaneously (s.c.) to all groups except C and CO to induce sensitization. Asthma was triggered via 1% OVA aerosol challenges on days 26-28. Over five days, OAF and OACRF received daily formoterol inhalations (50µg/kg), while OACR and OACRF were given chromium (400μg/kg). Treatments were timed to align with asthma induction protocols. Lipid profile and inflammatory indicators were examined at the end of the trial-Immunohistochemical analysis of lung tissue, Histopathological and lung tissue stained with Hematoxylin and Eosin. The combination therapy (OACRF) significantly reduced body weight (p<0.05), lowered LDL and triglycerides, increased HDL, and normalized lung tissue architecture compared to controls. Immunohistochemistry revealed reduced IL-1β and IL-17α expression. The (OACRF) group demonstrated superior asthma control by reducing body weight, improving inflammatory indicators, and restoring lung tissue to its normal state by administering chromium and formoterol therapy. The most effective strategy for treating both obesity and asthma is to address their two connected conditions. These findings demonstrate that combined chromium and formoterol therapy effectively addresses metabolic and inflammatory components of obesity-induced asthma, offering a promising dual-target therapeutic strategy.