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ORIGINAL RESEARCH article

Front. Pharmacol.
Sec. Ethnopharmacology
Volume 16 - 2025 | doi: 10.3389/fphar.2025.1512570

Montrichardia linifera (Arruda) Schott promotes accelerated wound healing in vitro: a promising healing

Provisionally accepted
Aline Bastos Aline Bastos 1,2Maurício Ferreira Gomes Maurício Ferreira Gomes 1,2Pinheiro, W. B. S. Pinheiro, W. B. S. 3Anderson de Santana Botelho Anderson de Santana Botelho 4Raimundo Junior da Rocha Batista Raimundo Junior da Rocha Batista 4Cristine Bastos do Amarante Cristine Bastos do Amarante 4Taia Maria Berto Rezende Taia Maria Berto Rezende 5André Khayat André Khayat 2,6Elizabeth S Yamada Elizabeth S Yamada 7Gilmara de Nazareth Tavares Bastos Gilmara de Nazareth Tavares Bastos 8*
  • 1 Laboratory of Neuroinflammation, Institute of Biological Sciences, Federal University of Pará, Belem, Brazil
  • 2 Oncology Research Center, Federal University of Pará, Belém, Brazil, Belém, Brazil
  • 3 Laboratory of Central Extraction, Institute of Exact and Natural Sciences, Federal University of Pará, Belem, Brazil
  • 4 Laboratory of Chemical Analysis, Coordination of Earth Sciences and Ecology, Emílio Goeldi Museum, Belem, Brazil
  • 5 Faculty of Health Sciences, University of Brasília, Brasília, Distrito Federal, Brazil
  • 6 Institute of Biological Sciences, Federal University of Pará, Belém, Brazil, Belém, Brazil
  • 7 Laboratory of Experimental Neuropathology, Institute of Biological Sciences, Federal University of Pará, Belem, Brazil
  • 8 Federal University of Pará, Belém, Brazil

The final, formatted version of the article will be published soon.

    Introduction: Montrichardia linifera (Arruda) Schott (M. linifera) is commonly used by Amazonian riverine communities for the treatment of skin ulcers, although its effects as a wound healer have never been evaluated until now. Therefore, the in vitro wound-healing activity of the extracts from the stem and petiole of M. linifera was investigated for the first time. Methods: The extracts were characterized by chromatography coupled with spectroscopic or spectrometric methods (HPTLC-UV and UHPLC-MS), and free radical scavenging was verified using bioautography with the DPPH radical. Cytotoxicity was evaluated through the MTT method, and a scratch assay was employed to assess cell migration, while in vitro cell proliferation was evaluated through immunofluorescence for BrdU-positive cells. Results: Chemical characterization revealed the presence of 13 metabolites in ESML and EPML extracts. Analytical analysis of the extract demonstrated the elimination of free radicals by autobiography. The extracts did not demonstrate cytotoxicity in fibroblasts and cell migration and proliferation were, significantly, increased reducing the wound area in vitro. Conclusion: Thus, it was observed that the extracts from the stem and petiole of M. linifera possess potential wound-healing effects in fibroblasts in vitro. This is a pioneering study that provides insights for future studies on the mechanisms of action of this species, in addition to validating the ethnopharmacological knowledge of this species used in the Amazon.

    Keywords: Montrichardia linifera1, healing2, Skin injury3, natural product4, wound5

    Received: 16 Oct 2024; Accepted: 07 Jan 2025.

    Copyright: © 2025 Bastos, Gomes, W. B. S., Botelho, Batista, Amarante, Rezende, Khayat, Yamada and Bastos. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Gilmara de Nazareth Tavares Bastos, Federal University of Pará, Belém, Brazil

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.