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METHODS article
Front. Pharmacol.
Sec. Ethnopharmacology
Volume 16 - 2025 | doi: 10.3389/fphar.2025.1506885
This article is part of the Research Topic Plant-derived Therapeutics and Traditional Medicine: Innovations, Challenges, and Opportunities in Breast Cancer Treatment View all 6 articles
Mechanism of Total Saponins of Ranunculus ternatus Thunb. in Treatment of Breast Cancer based on Liquid Chromatography-Mass Spectrometry and Network Pharmacology Findings
Provisionally accepted- Henan University of Chinese Medicine, Zhengzhou, China
To explore the mechanism of total saponins of Ranunculus ternatus Thunb. (RT) in the treatment of breast cancer (BC) using liquid chromatography-mass spectrometry (LC-MS) technology and network pharmacology.The active components of RT were detected using LC-MS. Components and targets of RT and BC were identified in different databases, and potential targets and pathways were predicted using protein-protein interaction network and pathway enrichment analyses. A mouse model of BC created by cellular injection and MCF-7 cells were used as research objects for in vivo and in vitro validation experiments to study the anti-BC mechanism of RT.A Kyoto Encyclopedia of Genes and Genomes analysis showed that the Janus kinase/signal transducer and activator of transcription signaling pathway might be associated with the anti-BC effects of RT. The in vivo and in vitro experiments showed that Total saponins from RT had a good anti-BC effect that can inhibit the expression of JAK2 and STAT3-related proteins and mRNA, affect the expression levels of serum inflammatory factors tumor necrosis factor-α, interleukin-6, and interleukin-10, inhibit tumor growth, proliferation, and migration, and promote tumor cell apoptosis.Total saponins from RT may play a role in BC treatment by regulating the JAK2/STAT3 signaling pathway.
Keywords: Total saponins of Ranunculus ternatus Thunb., breast cancer, Inflammation, JAK2/STAT3, signaling pathway
Received: 06 Oct 2024; Accepted: 06 Mar 2025.
Copyright: © 2025 Zhang, Nan, Zhao, Xie, Miao and Miao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Mingsan Miao, Henan University of Chinese Medicine, Zhengzhou, China
Jinxin Miao, Henan University of Chinese Medicine, Zhengzhou, China
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