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ORIGINAL RESEARCH article
Front. Pharmacol.
Sec. Ethnopharmacology
Volume 16 - 2025 | doi: 10.3389/fphar.2025.1498358
Effect of sheep placenta extract on D-galactose induced aging mouse
Provisionally accepted
Shan He 1
Yue Wu 1 Kaixian Lu 1 Heng Zhu 1 Xuan Wang 1 Yaoyao Qin 1
Huan Li 1
Lin Zeng 1 Jiaojiao Han 2 Xiangyang Zhou 3 Bin Zhang 1*
Bo Tang 1*- 1 Bengbu University, Bengbu, China
- 2 School of Marine Sciences, Ningbo University, Ningbo, Zhejiang Province, China
- 3 Genepioneer Biotechnologies Co, Ltd., Nanjing, Jiangsu Province, China
Sheep placenta extract (SPE), a representative traditional medicinal substance, exhibits multiple experimentally validated physiological properties, including anti-aging effects, wound healing acceleration, antioxidant activity, and anti-inflammatory mechanisms. However, the mechanism by which SPE influences the delay of aging is still not yet clear. Therefore, the aim of this study is to discuss the effects of SPE on aging in a D-galactose (D-gal) induced aging mouse model. In the serum of aging mice treated with SPE, the levels of antioxidant function such as superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) were notably higher compared to the blank group, whereas malondialdehyde (MDA) levels decreased. We revealed that SPE improved the changes in gut microbiota caused by aging in mice, with a significant decrease in the Firmicutes/Bacteroidetes (F/B) ratio in the gut. Furthermore, Akkermansia muciniphila (A. muciniphila), which is known for its regulating immune response and potential anti-aging effects, showed a significant increase of 1177.94%. This suggests that SPE's role in ameliorating aging may be associated with changes in the gut microbiota. The analysis of UHPLC-QE-MS combined with orthogonal partial least squares discriminant analysis (OPLS-DA) screening of differential metabolites in mouse serum metabolic profiles revealed a significant upregulation of 8,11,14,17eicosapentaenoic acid (EPA) and Triptolide in serum metabolites following SPE treatment, which are commonly believed to have immunosuppressive, anti-inflammatory, anti-proliferative, and anti-tumor effects. This suggests that these two metabolites may accumulate because of important biological functions and responses associated with the treatment. Therefore, we suggested that the extract of Hu sheep placenta can effectively enhance the antioxidant capacity of aging mice and improve the structure of intestinal microbiota.
Keywords: sheep placenta extract, antioxidant, D-galactose, Antiaging, Gut Microbiota
Received: 18 Sep 2024; Accepted: 21 Feb 2025.
Copyright: © 2025 He, Wu, Lu, Zhu, Wang, Qin, Li, Zeng, Han, Zhou, Zhang and Tang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Bin Zhang, Bengbu University, Bengbu, China
Bo Tang, Bengbu University, Bengbu, China
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