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ORIGINAL RESEARCH article

Front. Pharmacol.
Sec. Experimental Pharmacology and Drug Discovery
Volume 15 - 2024 | doi: 10.3389/fphar.2024.1532610
This article is part of the Research Topic Promising Photosensitive Agents for Photodynamic Therapy View all 5 articles

A telomere-related signature for predicting prognosis and immune microenvironment of osteosarcoma

Provisionally accepted
Shihao Li Shihao Li Lina Zhang Lina Zhang Haiyang Zhang Haiyang Zhang *
  • Zibo Central Hospital, Shandong, China

The final, formatted version of the article will be published soon.

    OBJECTIVE: Osteosarcoma is the most common primary bone cancer with a high propensity for local invasion and metastasis. Increasing research shows that telomeres play an important role in the occurrence and development of cancer. Thus, we established a telomere-related signature in osteosarcoma to comprehensively evaluate the pathogenic roles of telomeres in osteosarcoma. METHODS: The data of osteosarcoma were collected from TARGET and Gene Expression Omnibus database databases. Firstly, we constructed a telomere-related signature by univariate and LASSO Cox regression analyses. Subsequently, we analyzed the prognostic value, functional annotation, immune microenvironment, and cell communication pattern of telomere-related signature in osteosarcoma via comprehensive bioinformatics analysis. Cell proliferation was analyzed using CCK-8 assay, and the migration and invasion capabilities of the cells were evaluated using Transwell assay. RESULTS: Based on the SP110, HHAT, TUBB, MORC4, TERT, PPARG, MAP3K5, PAGE5, MAP7, CAMK1G, a telomere-related signature was built in osteosarcoma patients. The telomere-related signature could effectively predict the prognosis of osteosarcoma patients. The osteosarcoma patients in high TELscore group exhibited inferior prognosis. In addition, the telomere-related signature had predictive value in the immune microenvironment, drug sensitivity of osteosarcoma. Finally, we discovered significant reduction in MAP7 expression in osteosarcoma cells, and patients with low MAP7 expression was associated with poor prognosis. Moreover, overexpression of MAP7 significantly reduced cell proliferation and the ability of cell migration and invasion in osteosarcoma cells. CONCLUSIONS: A telomere-related signature was constructed in osteosarcoma patients, that had predictive values in the prognosis, immune microenvironment and

    Keywords: Osteosarcoma, telomere-related signature, prognosis, immune microenvironment, drug sensitivity, MAP7

    Received: 22 Nov 2024; Accepted: 31 Dec 2024.

    Copyright: © 2024 Li, Zhang and Zhang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Haiyang Zhang, Zibo Central Hospital, Shandong, 255000, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.