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ORIGINAL RESEARCH article

Front. Pharmacol.
Sec. Ethnopharmacology
Volume 15 - 2024 | doi: 10.3389/fphar.2024.1510171

Evaluation of Antidiarrheal Activity of 80% Hydromethanolic Crude Extract and Solvent Fractions of Terminalia Brownii Fresen (Combretaceae) Leaves in Swiss Albino Mice

Provisionally accepted
Khalid Ibrahim Kassaw Khalid Ibrahim Kassaw 1*Mastayit Geta Mengistie Mastayit Geta Mengistie 2Jibril Seid Yesuf Jibril Seid Yesuf 2Dawit Zewdu Wondafrash Dawit Zewdu Wondafrash 3
  • 1 Samara University, Semera, Ethiopia
  • 2 University of Gondar, Gondar, Amhara, Ethiopia
  • 3 St. Paul's Hospital Millennium Medical College, Addis Ababa, Addis Ababa, Ethiopia

The final, formatted version of the article will be published soon.

    Background: Although diarrhea is a preventable disease, it continues to have a significant impact on global health, with the burden being much greater in Sub-Saharan Africa. Medicinal plants represent affordable and locally available resources to address many diseases, including diarrhea.Thus, this study was aimed to investigate the antidiarrheal activities of the 80% hydromethanolic crude extract and solvent fractions of Terminalia brownii Fresen (Combretaceae) leaves in Swiss Albino mice.Methods: This study was carried out by administering 80% hydromethanolic crude extract and solvent fractions from the crude extract including n-hexane fraction, ethyl acetate fraction, and aqueous fraction at doses of 100 mg/kg, 200 mg/kg, and 400 mg/kg to the mice. The effects of these extracts and solvent fractions on reducing/delaying diarrhea were compared with the impact of the solvent used for reconstitution and a standard drug (loperamide 3 mg/kg or atropine 5 mg/kg), as well as to each other.The hydromethanolic crude extract and ethyl acetate fraction at all tested doses significantly reduced wet defecation (P<0.05). Besides, total defecation was significantly reduced at a dose of 200 mg/kg (P<0.05) and 400 mg/kg (P<0.001) of crude extract and ethyl acetate fraction at all tested doses (P<0.01. The aqueous fraction at 200 mg/kg and 400 mg/kg and the nhexane fraction at 400 mg/kg (P<0.05) significantly inhibit wet and total defecation. Likewise, in the enteropooling test, the crude extract and ethyl acetate fractions saw a significant reduction in weight and volume of intestinal contents at a dose of 200 mg/kg (P<0.05) and 400 mg/kg (P<0.05).Castor oil-induced intestinal motility was significantly reduced (p < 0.001) by the crude extract and ethyl acetate fractions at all tested doses.The result from this study suggests significant antidiarrheal activity of activity Terminalia brownii leaves which validates its traditional use. The 80% hydromethanolic crude extract and ethyl Acetate solvent fractions of Terminalia brownii Fresen leaves have shown better antidiarrheal activity.

    Keywords: Antidiarrheal activity, Terminalia brownii Fresen, Combretaceae, Castor oil-induced diarrhea. Hydromethanolic crude extract, Castor oil-induced diarrhea., hydromethanolic crude extract

    Received: 22 Oct 2024; Accepted: 23 Dec 2024.

    Copyright: © 2024 Kassaw, Mengistie, Yesuf and Wondafrash. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Khalid Ibrahim Kassaw, Samara University, Semera, Ethiopia

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.