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ORIGINAL RESEARCH article
Front. Pharmacol.
Sec. Cardiovascular and Smooth Muscle Pharmacology
Volume 15 - 2024 |
doi: 10.3389/fphar.2024.1506401
Mechanism of mTOR/RILP-regulated autophagic flux in increased susceptibility to myocardial ischemia-reperfusion in diabetic mice
Provisionally accepted
JIYAO ZHAO
1
Wei Shi
2
Yan Zheng
1
Junjie Wang
2
Muzhao Yuan
1
Yultuz Anwar
1
Yuxuan He
1
Hai Ping Ma
1*
Jianjiang Wu
1*- 1 Department of Anesthesiology, First Affiliated Hospital of Xinjiang Medical University, Urumqi, China
- 2 Changji Prefecture People's Hospital, Xinjiang, Changji, China
Background. The increased myocardial vulnerability that occurs in diabetic patients following an ischemia-reperfusion injury (I/RI) represents a significant perioperative safety risk. A comprehensive understanding of the intrinsic mechanisms underlying this phenomenon is therefore of paramount importance. Purposes. The objective of this study is to investigate the potential mechanism of action between impaired autophagic flux and increased vulnerability in diabetic myocardium. This will provide a foundation for the clinical search for effective preventive and curative measures. Methods. The transcriptomic alterations in autophagy-related genes following myocardial exposure to I/RI were analyzed by single-cell sequencing. This was followed by the validation of potential mechanisms of action between impaired autophagic flux and increased susceptibility at the cellular and animal levels, respectively.Results. After I/RI in diabetic myocardium, there was a significant increase in the number of CM1 subgroups and a specific down-regulation of 239 autophagy-related genes led by RILP. HE staining revealed that myocardial injury was exacerbated in diabetic mice subjected to I/RI. Transmission electron microscopy revealed that the accumulation of autophagic vesicles in cardiomyocytes of diabetic mice resulted in impaired autophagic flux. qRT-PCR revealed that the expression of RILP was significantly reduced in diabetic mice subjected to I/RI. WB showed that P62 was significantly increased and RILP was significantly decreased in diabetic mice subjected to I/RI compared to healthy mice. Inhibition of mTOR during hypoxia/reoxygenation (H/R) injury restored RILP expression and attenuated cellular injury in cardiomyocytes cultured with high glucose. Conclusion. Following I/RI in diabetic myocardium, an increase in the CM1 subpopulation and a reduction in RILP expression result in impaired autophagic flux. Regulation of the mTOR/RILP pathway can restore impaired autophagic flux and improve myocardial vulnerability, thereby exerting cardioprotective effects.
Keywords: Diabetic cardiomyopathy, Autophagic Flux, Vulnerability, mTOR/RILP pathway, Myocardial protection
Received: 05 Oct 2024; Accepted: 23 Dec 2024.
Copyright: © 2024 ZHAO, Shi, Zheng, Wang, Yuan, Anwar, He, Ma and Wu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Hai Ping Ma, Department of Anesthesiology, First Affiliated Hospital of Xinjiang Medical University, Urumqi, China
Jianjiang Wu, Department of Anesthesiology, First Affiliated Hospital of Xinjiang Medical University, Urumqi, China
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