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ORIGINAL RESEARCH article

Front. Pharmacol.
Sec. Ethnopharmacology
Volume 15 - 2024 | doi: 10.3389/fphar.2024.1501744
This article is part of the Research Topic Prevention and Treatment of Infectious Diseases by Herbal Medicine View all 7 articles

Panzerina lanata accelerates methicillin-resistant Staphylococcus aureus eradication by promoting migration and activation of neutrophils

Provisionally accepted
Dan Zhu Dan Zhu *Shuai Dong Shuai Dong Xingyuan Bai Xingyuan Bai *Bin Chen Bin Chen Minzhe Fan Minzhe Fan *Qi Liu Qi Liu *Yubo Zhao Yubo Zhao *Linsen Li Linsen Li *
  • Minzu University of China, Beijing, China

The final, formatted version of the article will be published soon.

    Background: Panzerina lanata (Lanata) is generally used to treat pustule infection in Inner Mongolia folk medicine and is called "the holy medicine for pustule". However, the pharmacological mechanism of Lanata in treating pustule infection is still unclear.Aims: This study aimed to investigate the therapeutic effects of Lanata on skin infection and explore the underlying mechanisms.A skin wound methicillin-resistant Staphylococcus aureus (MRSA) infection mouse model was established to evaluate the healing effect of Lanata on infected wounds. In vitro assays were also conducted to determine the antibacterial activity of Lanata. Flow cytometry and immunohistochemistry were used to dynamically detect the number of neutrophils in bone marrow, peripheral blood and MRSA infected wound. Protein expression in the infected wound skin was detected by protein chip. Using an air pouch MRSA infection mouse model, the number of neutrophils, reactive oxygen species (ROS) level in neutrophils, and neutrophil extracellular traps (NETs) formation were dynamically detected by flow cytometry and immunofluorescence. RNA-seq, RT-qPCR, flow cytometry, ELISA, and CXC chemokine receptor 2 (CXCR2) and P-selectin glycoprotein ligand-1 (PSGL-1) inhibitors were used to explore the mechanism of Lanata in regulating neutrophils.In vitro assays showed that Lanata had no direct antibacterial activity. In skin wound MRSA infected mouse, Lanata promoted the rapid migration of neutrophils from bone marrow via peripheral blood to the wound site to eradicate MRSA in the acute stage of infection and accelerate wound healing. Skin protein chip analysis showed that Lanata upregulated CXCR2 and PSGL-1 protein levels in skin wounds. Further, analysis using the air pouch MRSA infection mouse model found that Lanata not only promoted the rapid migration of neutrophils from peripheral blood to the air pouch, but also enhanced the activation of neutrophils, including the increase of ROS and the release of NETs, and upregulated the expression of CXCR2, PSGL-1 and myeloperoxidase (MPO) in neutrophils. Inhibition of CXCR2 and MPO significantly attenuated the effect of Lanata on promoting migration and activation of neutrophils.Panzerina lanata resists MRSA infection by promoting migration and activation of neutrophils to rapidly eradicate MRSA.

    Keywords: ABAH, 4-aminobenzoic hydrazide, CXCR2, CXC chemokine receptor 2, DEGs, differentially expressed genes, DEPs, differentially expressed proteins, H&E, hematoxylin and eosin, MFI, mean fluorescence intensity, MPO, myeloperoxidase, MRSA, methicillin-resistant Staphylococcus aureus

    Received: 25 Sep 2024; Accepted: 16 Dec 2024.

    Copyright: © 2024 Zhu, Dong, Bai, Chen, Fan, Liu, Zhao and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Dan Zhu, Minzu University of China, Beijing, China
    Xingyuan Bai, Minzu University of China, Beijing, China
    Minzhe Fan, Minzu University of China, Beijing, China
    Qi Liu, Minzu University of China, Beijing, China
    Yubo Zhao, Minzu University of China, Beijing, China
    Linsen Li, Minzu University of China, Beijing, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.