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ORIGINAL RESEARCH article

Front. Pharmacol.
Sec. Inflammation Pharmacology
Volume 15 - 2024 | doi: 10.3389/fphar.2024.1500617
This article is part of the Research Topic The Mechanisms of Fibrotic Disorders and Pharmacological Therapies View all 9 articles

VASN knockout induced myocardial fibrosis by downregulating non collagen fibers and promoting inflammation in mice

Provisionally accepted
Junming Sun Junming Sun *Siwei Yin Siwei Yin Qiurui Li Qiurui Li Jun Zhang Jun Zhang Xiaoping Guo Xiaoping Guo Na Yu Na Yu Bing Hu Bing Hu Yiqiang Ouyang Yiqiang Ouyang Qiaojuan Huang Qiaojuan Huang Min He Min He
  • Guangxi Medical University, Nanning, China

The final, formatted version of the article will be published soon.

    Myocardial fibrosis (MF) is an important cause of heart failure and cardiac arrest.VASN deletion (VASN -/-) leads to pathological cardiac hypertrophy; however, it is not yet clear whether pathological myocardial hypertrophy transitions to myocardial fibrosis in mice. VASN-knockout mice showed typical pathological, imaging, and molecular features of MF on HE staining, Masson staining, Sirius red staining, Q-PCR, IHC-P, and IF. RNA was extracted from mouse heart tissue, identified, and sequenced in vitro. Differential analysis of genes found that ECM genes (COL6A1, COL9A1, and FRAS1) showed a strong correlation, while their expression levels were significantly reduced by Q-PCR, IHC-P, and WB. The expression levels of ECM genes were significantly reduced, but the expression levels of inflammatory factors (IL1β, and IL6) were significantly upregulated in the heart tissue of VASN-knockout mice. These results preliminarily revealed that VASN knockout induced myocardial fibrosis by regulating non-collagen fibers and inflammation.

    Keywords: Myocardial fibrosis, vasorin, non collagen fibers, Inflammation, Mice

    Received: 23 Sep 2024; Accepted: 02 Dec 2024.

    Copyright: © 2024 Sun, Yin, Li, Zhang, Guo, Yu, Hu, Ouyang, Huang and He. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Junming Sun, Guangxi Medical University, Nanning, China

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