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ORIGINAL RESEARCH article

Front. Pharmacol.
Sec. Ethnopharmacology
Volume 15 - 2024 | doi: 10.3389/fphar.2024.1500085
This article is part of the Research Topic Traditional Medicines in the Treatment of Infectious Diseases – Challenges and advances View all 4 articles

Exploration of the Anti-inflammatory Potential of the Polygonum bistorta L.: Protection Against LPS-Induced Acute Lung Injury in Rats via NF-ĸβ Pathway Inhibition

Provisionally accepted
Sajida Perveen Sajida Perveen 1*Kashif ur Rehman Khan Kashif ur Rehman Khan 2Shahid Muhammad Iqbal Shahid Muhammad Iqbal 1Hanan Y. Aati Hanan Y. Aati 3Areej M. Al-taweel Areej M. Al-taweel 3Liaqat Hussain Liaqat Hussain 4Musaddique hussain Musaddique hussain 5
  • 1 Department of Pharmacology, Faculty of Pharmacy, Islamia University of Bahawalpur, Bahawalpur, Punjab, Pakistan
  • 2 Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Islamia University of Bahawalpur, Bahawalpur, Punjab, Pakistan
  • 3 Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia
  • 4 Faculty of Pharmaceutical Sciences, Government College University Faisalabad, Faisalabad, Punjab, Pakistan
  • 5 Department of Medicine, Heersink School of Medicine, University of Alabama at Birmingham, Birmingham, Alabama, United States

The final, formatted version of the article will be published soon.

    Traditional medicine uses the roots and rhizomes of Polygonum bistorta L. (Polygonaceae) to treat cough, bronchitis, and other respiratory infections. Our goal was to gain insights into the lung protective effects of the roots of Polygonum bistorta L. against lipopolysaccharide-induced acute lung injury in rats along with the possible mechanism(s). The outcomes revealed deliberate quantities of total phenolic and flavonoid contents of 156.2±5.13 GAE/g and 179.45 ± 2.08 mg QE/g, respectively. Crude extract possesses a maximum inhibitory potential of 81.77±0.62% for acetylcholinesterase against Eserine. Acute oral toxicity study revealed LD 50 beyond 7 g/kg.Plant extract markedly restored LPS-induced hypoxemia, pulmonary edema, histopathological alterations, and leukocyte infiltration in the lung. ELISA testing on BALF expressed that the plant extract efficiently reinstated superoxide dismutase, total anti-oxidant capacity, melondialdehyde, and total oxidative stress. qRT-PCR indicated a decline in endotoxin-induced overproduction of pro-inflammatory markers, oxidative stress, transcription factor, and down regulated antioxidant potential in extract-treated groups. Furthermore, 24 metabolites were identified and quantified via GC-MS. A molecular docking procedure was implemented on the bioactive metabolites that were identified to evaluate their potential for inhibiting AChE. In conclusion, Polygonum bistorta roots mitigate inflammation and oxidative stress by improving redox signaling, and NF-ĸβ (p65) pathways. So, this can play a role in strategies for overcoming therapeutic challenges.

    Keywords: Polygonum bistorta, Polygonaceae, NF-ĸβ, Gene Expression, medicinal plants, molecular docking

    Received: 22 Sep 2024; Accepted: 16 Dec 2024.

    Copyright: © 2024 Perveen, Khan, Iqbal, Aati, Al-taweel, Hussain and hussain. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Sajida Perveen, Department of Pharmacology, Faculty of Pharmacy, Islamia University of Bahawalpur, Bahawalpur, Punjab, Pakistan

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.