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ORIGINAL RESEARCH article

Front. Pharmacol.
Sec. Gastrointestinal and Hepatic Pharmacology
Volume 15 - 2024 | doi: 10.3389/fphar.2024.1498625

Polysaccharides Derived from Golden Mushroom (Cantharellus cibarius Fr.) Modulate Gut Microbiota and Enhance Intestinal Barrier Function to Ameliorate Dextran Sulfate Sodium-Induced Colitis in Mice

Provisionally accepted
Yamina ALIOUI Yamina ALIOUI 1,2Hidayat Ullah Hidayat Ullah 1,2Sharafat Ali Sharafat Ali 1,3*Mujeeb Ur Rahman Mujeeb Ur Rahman 1,2*Maroua Elkharti Maroua Elkharti 1,3*Nabeel Ahmed Farooqui Nabeel Ahmed Farooqui 1,2Ata Ur Rehman Ata Ur Rehman 4Muhammad Ilyas Muhammad Ilyas 1,2*Duaa M Alsholi Duaa M Alsholi 5*Nimra Zafar Siddiqui Nimra Zafar Siddiqui 1,2Muhsin Ali Muhsin Ali 1,2Liang Wang Liang Wang 6*Yi Xin Yi Xin 2*
  • 1 Dalian Medical University, Dalian, China
  • 2 Department of Biotechnology, College of Basic Medical Sciences, Dalian Medical University, Dalian, Liaoning Province, China
  • 3 Department of Biochemistry and Molecular Biology, College of Basic Medical Science, Dalian Medical University, Dalian 116044, China., Dalian, China
  • 4 Multidisciplinary Neuroprotection Laboratories, Duke University School of Medicine, Durham, NC 27708, USA, Durham, United States
  • 5 Department of Allied Medical Sciences, Zarqa University, Zarqa, Zarqa, Jordan
  • 6 5Stem Cell Clinical Research Center, National Joint Engineering Laboratory, Regenerative Medicine Center, The First Affiliated Hospital of Dalian Medical University, Dalian 116011, China, First Affiliated Hospital, Dalian Medical University, Dalian, Liaoning Province, China

The final, formatted version of the article will be published soon.

    Inflammatory bowel disease (IBD), including ulcerative colitis, is marked by intestinal barrier disruptions, immune system dysregulation, and an imbalance in the gut microbiota. The golden chanterelle mushroom, Cantharellus cibarius Fr., a popular edible mushroom, has shown potential therapeutic benefits. This study examines the therapeutic potential of a crude polysaccharide extract obtained from C. cibarius Fr. (CCP) on intestinal barrier integrity, inflammatory cytokine levels, and gut microbiota composition in a murine model of colitis induced by dextran sulfate sodium (DSS). To induce colitis BALB/c mice were provided to consume autoclaved water with 3% DSS for 7 days, followed by 14 days of CCP supplementation. Colon was observed histologically, gene expression, inflammatory responses, tight junction proteins expression, gut barrier integrity, and cytokines levels were measured and 16S rRNA were evaluated. CCP treatment alleviates colitis symptoms by improving body weight, tight junction protein expression, and enhancing intestinal integrity through increased mucin-2 and tight junction protein expression.Additionally, CCP administration regulates the altered immune response by mitigating the expression of pro-inflammatory cytokines and upregulating anti-inflammatory cytokines.Furthermore, CCP supplementation effectively modulates DSS-induced dysbiosis as demonstrated by 16S rRNA sequencing results. These results highlight that crude polysaccharides from the golden chanterelle mushroom, C. cibarius Fr., hold promise for treating colitis, via strengthening the intestinal barrier, regulating inflammatory responses, and reshaping the gut dysbiosis in a DSS-induced colitis model. CCP offers a novel approach for managing colitis, as a chronic inflammatory condition.

    Keywords: Cantharellus cibarius Fr., Polysaccharides, Gut Microbiota, inflammatory bowel disease, Dextran sulfate sodium, intestinal barrier

    Received: 20 Sep 2024; Accepted: 27 Nov 2024.

    Copyright: © 2024 ALIOUI, Ullah, Ali, Rahman, Elkharti, Farooqui, Rehman, Ilyas, Alsholi, Siddiqui, Ali, Wang and Xin. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Sharafat Ali, Dalian Medical University, Dalian, China
    Mujeeb Ur Rahman, Dalian Medical University, Dalian, China
    Maroua Elkharti, Dalian Medical University, Dalian, China
    Muhammad Ilyas, Dalian Medical University, Dalian, China
    Duaa M Alsholi, Department of Allied Medical Sciences, Zarqa University, Zarqa, 13110, Zarqa, Jordan
    Liang Wang, 5Stem Cell Clinical Research Center, National Joint Engineering Laboratory, Regenerative Medicine Center, The First Affiliated Hospital of Dalian Medical University, Dalian 116011, China, First Affiliated Hospital, Dalian Medical University, Dalian, 116011, Liaoning Province, China
    Yi Xin, Department of Biotechnology, College of Basic Medical Sciences, Dalian Medical University, Dalian, 116044, Liaoning Province, China

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