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ORIGINAL RESEARCH article

Front. Pharmacol.
Sec. Ethnopharmacology
Volume 15 - 2024 | doi: 10.3389/fphar.2024.1492098

Macranthoidin B restrains the epithelial-mesenchymal transition through COX-2/PGE2 pathway in endometriosis

Provisionally accepted
Yi Ding Yi Ding 1,2Xiaoqian Yang Xiaoqian Yang 2Qinghua Wei Qinghua Wei 2Xuanming Bi Xuanming Bi 2Yuxin Zhang Yuxin Zhang 2Yuxia Ma Yuxia Ma 2Meisen Yang Meisen Yang 3Xiaoyu Xu Xiaoyu Xu 2Cong Li Cong Li 4*Qin Wang Qin Wang 5*Yi Chen Yi Chen 1*
  • 1 Chongqing University of Chinese Medicine, Chongqing, China
  • 2 Southwest University, Chongqing, China
  • 3 Traditional Chinese Medicine Industry Center of Xiushan Tujia & Miao Autonomous County, Chongqing, China
  • 4 First Affiliated Hospital of Chongqing Medical University, Chongqing, Chongqing Municipality, China
  • 5 Chongqing Hospital of Traditional Chinese Medicine, Chongqing, China

The final, formatted version of the article will be published soon.

    Macranthoidin B is one of the primary and unique triterpenoid saponin metabolites from Lonicera macranthoides Hand. -Mazz, which is used to treat endometriosis (EMS) in traditional Chinese medicine. However, the effect of macranthoidin B remains unknown in EMS. This study aimed to elucidate the effect and mechanism of macranthoidin B in EMS. In a rat autograft EMS model, macranthoidin B suppressed ectopic lesion volume, improved histopathological morphology, and regulated serum estradiol (E2) and progesterone (PROG) levels. Additionally, macranthoidin B inhibited invasion and metastasis of primary endometriotic stromal cells and HEC1-B cells. Mechanistically, macranthoidin B suppressed COX-2/PGE2 pathway and epithelial-mesenchymal transition both in vivo and in vitro. LPS, the COX-2/PGE2 pathway activator, showed the promotion of epithelial-mesenchymal transition, invasion and metastasis. Macranthoidin B exhibited the antagonistic effects against LPS. Celecoxib, the COX-2/PGE2 pathway inhibitor, restrained the epithelial-mesenchymal transition, invasion and metastasis. This effect of celecoxib was enhanced by macranthoidin B.Macranthoidin B prevents epithelial-mesenchymal transition through COX-2/PGE2 pathway in EMS. It will facilitate the macranthoidin B's development and broaden its potential application.

    Keywords: Macranthoidin B, Endometriosis, COX-2/PGE2 pathway, Epithelial-Mesenchymal Transition, invasion and metastasis

    Received: 06 Sep 2024; Accepted: 29 Nov 2024.

    Copyright: © 2024 Ding, Yang, Wei, Bi, Zhang, Ma, Yang, Xu, Li, Wang and Chen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Cong Li, First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, Chongqing Municipality, China
    Qin Wang, Chongqing Hospital of Traditional Chinese Medicine, Chongqing, 400021, China
    Yi Chen, Chongqing University of Chinese Medicine, Chongqing, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.