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ORIGINAL RESEARCH article

Front. Pharmacol.
Sec. Gastrointestinal and Hepatic Pharmacology
Volume 15 - 2024 | doi: 10.3389/fphar.2024.1485035

Integrated multi-omics analyses discovered that cis-TSG alleviated liver injury via modulating lipid metabolism

Provisionally accepted
Tekleab T. Teklehaimanot Tekleab T. Teklehaimanot 1*Jiang Wu Jiang Wu 2Patrick K. Oduro Patrick K. Oduro 3Ze Li Ze Li 1Chenxi Wang Chenxi Wang 1Hao Chen Hao Chen 1Lin Zhang Lin Zhang 1Haitao Wang Haitao Wang 1Liming Wang Liming Wang 1Lifeng Han Lifeng Han 1
  • 1 Tianjin University of Traditional Chinese Medicine, Tianjin, China
  • 2 Shenzhen Technology University, Shenzhen, Guangdong, China
  • 3 Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, New York, United States

The final, formatted version of the article will be published soon.

    Polygonum multiflorum shows dual hepatoprotective and hepatotoxic effects, necessitating detailed investigations into its bioactive components, particularly the stilbene glycosides, responsible for this duality effect. This study examined the pharmacological effects of cis-TSG, a stilbene glycoside, on ANIT-induced cholestasis in C57BL/6J mice. ANIT administration led to significant liver injury, as indicated by elevated ALT and AST levels and disrupted lipid metabolism. cis-TSG treatment significantly reduced ALT and AST levels, restored normal lipid profiles, and mitigated histological liver damage. Metabolomic and lipidomic analyses revealed that cis-TSG modulated key metabolic pathways, including glycerophospholipid metabolism, sphingolipid metabolism, and bile acid biosynthesis.Notably, cis-TSG increased levels of MUFAs and PUFAs, enhancing PPARα activation, which contributed to its hepatoprotective effects. Transcriptomic analysis supported these findings, showing downregulation of genes involved in lipid metabolism, inflammation, and oxidative stress in the cis-TSG-treated group. These results suggest that cis-TSG mitigates ANIT-induced cholestasis through the modulation of lipid metabolism and activation of PPARα. Our findings provide new insights into the therapeutic potential of cis-TSG for liver diseases and underscore the need for further research to fully elucidate its mechanisms of action.

    Keywords: cis-TSG, Stilbene glycoside, Cholestasis, Metabolomics, lipidomics, Transcriptomics

    Received: 23 Aug 2024; Accepted: 05 Nov 2024.

    Copyright: © 2024 Teklehaimanot, Wu, Oduro, Li, Wang, Chen, Zhang, Wang, Wang and Han. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Tekleab T. Teklehaimanot, Tianjin University of Traditional Chinese Medicine, Tianjin, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.