Skip to main content

ORIGINAL RESEARCH article

Front. Pharmacol.
Sec. Ethnopharmacology
Volume 15 - 2024 | doi: 10.3389/fphar.2024.1474706
This article is part of the Research Topic Food Derived Bioactive Metabolites: Unlocking their Potential Health Benefits and Medical Potential View all articles

First-time report on compound isolation from two Colocasia species: vegetable-derived bioactive metabolites and their medicinal potentials

Provisionally accepted
  • 1 Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka, Bangladesh
  • 2 University of Dhaka, Dhaka, Dhaka, Bangladesh
  • 3 International Islamic University, Chittagong, Chittagong, Chittagong, Bangladesh

The final, formatted version of the article will be published soon.

    Background: Colocasia affinis Schott and Colocasia gigantea Hook.f. are two commonly found vegetable species from Colocasia genus found mainly in the Asian region. Objectives: The objective of this study was to isolate bioactive phytochemicals from C affinis and C. gigantea and elucidate their structure employing NMR technique followed by bioactivity evaluation. Methodology: Column chromatography was utilized to isolate phytochemicals followed by NMR analysis for characterization. In-vivo analgesic test was performed through acetic acid-induced writhing test, anti-inflammatory through formalin-induced licking test and antidiarrheal through castor-oil induced diarrhea model. In-vitro antimicrobial test was executed through disc-diffusion method. Computer-aided simulation was also implemented to validate wet lab results. Results: Six compounds from C. affinis and C. gigantea were isolated and characterized from the dichloromethane (DCM) soluble fractions of the methanolic extracts of these two species. Three of the compounds were from C. gigantea and proposed as Penduletin (C1), a mixture of α-amyrin (C2a) and β-amyrin (C2b), and monoglyceride of stearic acid (C3) while rest of the three compounds were from C. affinis and proposed as Penduletin (C4) (which was isolated from C. gigantea also), 7,8- (3'',3''-dimethyl-pyrano)-4′-hydroxy flavonol (C5), and lastly a mixture of 7,8- (3'',3''-dimethyl-pyrano)-4′-hydroxy flavonol (C5) and 4',7,8- trihydroxy flavonol (C6). These compounds showed promising zone of inhibitions against gram-positive, gram-negative bacteria and fungi. In the antidiarrheal test, C5 demonstrated the highest reduction in castor oil-induced diarrhea (44.44%) at 20 mg/kg dose compared to loperamide’s 77.78% reduction. However, analgesic assessment showed noteworthy inhibition of acetic acid-induced writhing by C1/C4, and C2 (56.52%) at 20 mg/kg dose compared to the 76.09% by diclofenac sodium while C2 showed pronounced anti-inflammatory action by 68.15% and 52.06% reduction respectively in early and later phase compared to the ibuprofen’s outcomes of 73.54% and 74.68%. Responsible targets such as dihydrofolate reductase (DHFR) for anti-microbial, kappa opioid receptor (KOR) for antidiarrheal, and cyclooxygenase 2 (COX-2) for anti-inflammatory and analgesic activities showed noteworthy binding affinity with isolated compounds while ADME/T studies displayed these phytochemicals’ drug-likeliness profiles. Conclusion: Finally, to the best of our knowledge this is the first-time report on compound isolation from these plants which demands further extensive research.

    Keywords: Colocasia gigantea, Colocasia affinis, vegetable, NMR, Antibacterial, Antidiarrheal, analgesic, anti-inflammatory

    Received: 02 Aug 2024; Accepted: 28 Oct 2024.

    Copyright: © 2024 Alam, Richi, Emon, Chowdhury, Hasan and Haque. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Safaet Alam, Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka, Bangladesh
    Abu Asad Chowdhury, University of Dhaka, Dhaka, 1000, Dhaka, Bangladesh
    Mohammad Rashedul Haque, University of Dhaka, Dhaka, 1000, Dhaka, Bangladesh

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.