AUTHOR=Zheng Xiaoyu , Zhang Hongwei , Zhang Yehao , Ding Zhao , Huang Zishan , Li Haoran , Yao Mingjiang , Song Wenting , Liu Jianxun 

TITLE=Salidroside ameliorates cerebral ischemic injury and regulates the glutamate metabolism pathway in astrocytes

JOURNAL=Frontiers in Pharmacology

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2024.1472100

DOI=10.3389/fphar.2024.1472100

ISSN=1663-9812

ABSTRACT=<sec><title>Background and Aim</title><p>Salidroside (SA) is the main active component of <italic>Rhodiola rosea</italic> L., with potential in treating cardiovascular and cerebrovascular diseases and cerebral ischemia. However, its efficacy and mechanism in cerebral ischemia remain unclear, particularly regarding its effect on glutamate (Glu) metabolism. In this paper, we aimed to investigate the efficacy of SA in treating cerebral ischemia and its pharmacological mechanism.</p></sec><sec><title>Experimental procedure</title><p>We studied the effects of SA on SD rats with cerebral ischemia, evaluating neurobehavior, cerebral water content, infarct size, and brain microstructure. We also assessed its impact on glial fibrillary acidic protein (GFAP), glutamine synthetase (GS), and glutamate transporter 1 (GLT-1) proteins using immunohistochemistry and Western blot. Additionally, we used SVGp12 cells to simulate cerebral ischemia and measured Glu levels and used Western blot to observe the level of GS and GLT-1.</p></sec><sec><title>Results</title><p>SA improved neural function, reduced infarct size, and regulated GSH and Glu levels in rats. In cell experiments, SA increased cell viability and decreased Glu concentration after ischemia induction. It also regulated the expression of GFAP, GS, and GLT-1.</p></sec><sec><title>Conclusion</title><p>SA alleviates cerebral ischemia-induced injury by acting on astrocytes, possibly through regulating the glutamate metabolic pathway.</p></sec>