AUTHOR=Raiten Jacob , Abd Genevieve M. , Handelsman Shane B. , Patel Harshank V. , Ku Jennifer C. , Parsons Agata M. , Wassink Jonathan L. , Hayes Sheridan L. , Overbay Juliana , Li Yong TITLE=Hypoxia-induced PD-L1 expression and modulation of muscle stem cell allograft rejection JOURNAL=Frontiers in Pharmacology VOLUME=15 YEAR=2024 URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2024.1471563 DOI=10.3389/fphar.2024.1471563 ISSN=1663-9812 ABSTRACT=
Stem cell therapy has shown immense promise in treating genetic disorders, particularly muscular diseases like Duchenne muscular dystrophy (DMD). This study investigates a novel method to enhance the viability of stem cell transplants in DMD by upregulating Programmed Death Ligand 1 (PD-L1) in muscle stem cells (MuSCs) through preconditioning with hypoxia and/or interferon-γ (IFN-γ) to mitigate T cell immune rejection. MuSCs were treated with 5% hypoxia for 72 h and further treated with IFN-γ to enhance PD-L1 expression. Additionally, gain and loss experiments using a PD-L1 inhibitor (BMS-1) were conducted to investigate cellular expression profiles