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ORIGINAL RESEARCH article
Front. Pharmacol.
Sec. Drug Metabolism and Transport
Volume 15 - 2024 |
doi: 10.3389/fphar.2024.1470377
Analysis of riociguat and desmethyl riociguat by UPLC-MS/MS and its interaction with quercetin
Provisionally accepted- 1 Ningbo Municipal Hospital of Traditional Chinese Medicine (TCM), Ningbo, China
- 2 First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, China
Riociguat, an orally soluble guanylate cyclase (sGC)-promoting drug, is mainly used in the clinical treatment of pulmonary hypertension (PH). In this study, a novel ultra-performance liquid chromatography-tandem mass spectrometry method was developed to quantify the concentrations of riociguat and its metabolite (M1) in plasma. The precision, stability, accuracy, matrix effect, and recovery of the methodology were satisfactory. Quercetin, a well-recognized compound, functions as a novel anticancer agent with the potential to alleviate symptoms of PH. Therefore, the potential interaction between quercetin and riociguat was investigated in this study.The levels of riociguat and M1 in rat plasma were measured using the method developed in this study to evaluate the interactions between riociguat and quercetin in rats. The results revealed that quercetin significantly inhibited riociguat and M1 metabolism with increased systemic exposure.
Keywords: Riociguat, desmethyl riociguat, Quercetin, UPLC-MS/MS, Drug-Drug Interaction
Received: 25 Jul 2024; Accepted: 05 Sep 2024.
Copyright: © 2024 Li, Chen, Zhang, Li and Lin. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Xiaohai Chen, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, Zhejiang Province, China
Siping Zhang, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, Zhejiang Province, China
Wanshu Li, Ningbo Municipal Hospital of Traditional Chinese Medicine (TCM), Ningbo, China
Hangjuan Lin, Ningbo Municipal Hospital of Traditional Chinese Medicine (TCM), Ningbo, China
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