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ORIGINAL RESEARCH article
Front. Pharmacol.
Sec. Ethnopharmacology
Volume 15 - 2024 |
doi: 10.3389/fphar.2024.1466578
This article is part of the Research Topic Plant Metabolites in Drug Discovery: The Prism Perspective between Plant Phylogeny, Chemical Composition, and Medicinal Efficacy, Volume III View all 3 articles
A comparative analysis of chloroplast genomes revealed the chloroplast heteroplasmy of Artemisia annua
Provisionally accepted
Ding Xiaoxia
1
Hengyu Pan
1*
Peiqi Shi
1*
Siyu Zhao
1*
Shengye Bao
1*
Shan Zhong
2*
Chunyan Dai
1*
Jieting Chen
1*
Lu Gong
1
Danchun Zhang
1
Xiaohui Qiu
1*
Baosheng Liao
1*
Zhihai Huang
1*- 1 Second Clinical Medical College, Guangzhou University of Traditional Chinese Medicine, Guangzhou, China
- 2 Mudanjiang Normal University, Mudanjiang, China
Artemisia annua L. is the main source of artemisinin, an antimalarial drug. High diversity of morphological characteristics and artemisinin contents of A. annua has affected the stable production of artemisinin while efficient discrimination method of A. annua strains is not available. The complete chloroplast (cp) genomes of 38 A. annua strains were assembled and analyzed in this study. Phylogenetic analysis of Artemisia species showed that distinct intraspecific divergence occurred in A. annua strains. A total of 38 A. annua strains were divided into two distinct lineages, one lineage containing widely-distributed strains and the other lineage only containing strains from northern China. The A. annua cp genomes ranged from 150, 953 to 150, 974 bp and contained 131 genes, and no presence or absence variation of genes was observed. The IRs and SC junctions were located in rps19 and ycf1, respectively, without IR contraction observed. Rich sequence polymorphisms were observed among A. annua strains, and a total of 60 polymorphic sites representing 14 haplotypes were identified which unfolding the cpDNA heteroplasmy of A. annua. In conclusion, this study provided valuable resource for A. annua strains identification and provided new insights into the evolutionary characteristics of A. annua.
Keywords: Artemisia annua, Chloroplast genome, genetic diversity, Strains identification, comparative analysis
Received: 18 Jul 2024; Accepted: 31 Jul 2024.
Copyright: © 2024 Xiaoxia, Pan, Shi, Zhao, Bao, Zhong, Dai, Chen, Gong, Zhang, Qiu, Liao and Huang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Hengyu Pan, Second Clinical Medical College, Guangzhou University of Traditional Chinese Medicine, Guangzhou, China
Peiqi Shi, Second Clinical Medical College, Guangzhou University of Traditional Chinese Medicine, Guangzhou, China
Siyu Zhao, Second Clinical Medical College, Guangzhou University of Traditional Chinese Medicine, Guangzhou, China
Shengye Bao, Second Clinical Medical College, Guangzhou University of Traditional Chinese Medicine, Guangzhou, China
Shan Zhong, Mudanjiang Normal University, Mudanjiang, China
Chunyan Dai, Second Clinical Medical College, Guangzhou University of Traditional Chinese Medicine, Guangzhou, China
Jieting Chen, Second Clinical Medical College, Guangzhou University of Traditional Chinese Medicine, Guangzhou, China
Xiaohui Qiu, Second Clinical Medical College, Guangzhou University of Traditional Chinese Medicine, Guangzhou, China
Baosheng Liao, Second Clinical Medical College, Guangzhou University of Traditional Chinese Medicine, Guangzhou, China
Zhihai Huang, Second Clinical Medical College, Guangzhou University of Traditional Chinese Medicine, Guangzhou, China
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