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ORIGINAL RESEARCH article

Front. Pharmacol.
Sec. Pharmacology of Anti-Cancer Drugs
Volume 15 - 2024 | doi: 10.3389/fphar.2024.1459520
This article is part of the Research Topic Multi-omics Application in Exploring Potential Biomarkers Targeting Resistance of Anti-Cancer Drugs View all 15 articles

Sorbaria sorbifolia flavonoid derivative induces mitochondrial apoptosis of human hepatoma cells through Bclaf1

Provisionally accepted
Jiaxin Chen Jiaxin Chen Haoyi Cheng Haoyi Cheng Chunhua Bai Chunhua Bai Dandan Wang Dandan Wang Jinghao Fu Jinghao Fu Jinge Hao Jinge Hao Yixuan Wang Yixuan Wang Zhang Xuewu Zhang Xuewu *
  • Yanbian University, Yanji, China

The final, formatted version of the article will be published soon.

    4',5,7-trihydroxy-8-methoxyflavone was anticancer monomer components isolated from the traditional Chinese medicine Sorbaria sorbifolia. 4 ' , 5-Dihydroxy-7-piperazinemethoxy-8methoxyflavonoids (DMF) with good solubility and anti-tumor effects was obtained by chemical modification in the early stage. This study explored the mechanism by which DMF regulates mitochondrial apoptosis of human hepatoma cells through Bcl-2-associated transcription factor 1 (Bclaf1). DMF inhibited the proliferation of human hepatoma cells in a concentration-and timedependent manner, and DMF induced cells mitochondrial apoptosis. Molecular docking and cell assay results demonstrated that DMF inhibits Bclaf1 expression by binding to the active site of Bclaf1. Lentivirus transfection was used to construct cells with stable knockout and overexpression of Bclaf1 and a Hep3B xenograft model was constructed in nude mice, the mechanism by which DMF induced mitochondrial apoptosis of human hepatoma cells through Bclaf1 was further verified in vitro and in vivo. These findings indicated that DMF induced human hepatoma cells mitochondrial apoptosis through Bclaf1.

    Keywords: Sorbaria sorbifolia, 4', 5-Dihydroxy-7-piperazinemethoxy-8-methoxyflavonoids, liver cancer, Bcl-2-associated transcription factor 1, Mitochondrial apoptosis

    Received: 04 Jul 2024; Accepted: 30 Aug 2024.

    Copyright: © 2024 Chen, Cheng, Bai, Wang, Fu, Hao, Wang and Xuewu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Zhang Xuewu, Yanbian University, Yanji, China

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