Junxuan Li
Jiaxin Ding
*
Li Fu
*The final, formatted version of the article will be published soon.
ORIGINAL RESEARCH article
Front. Pharmacol.
Sec. Integrative and Regenerative Pharmacology
Volume 15 - 2024 |
doi: 10.3389/fphar.2024.1453036
This article is part of the Research Topic Integrative Pharmacological Approaches for Regenerating Cartilage and Bone Tissue View all 6 articles
A Novel Functionally Graded Bilayer Membrane with Excellent Barrier Function and In Vivo Osteogenesis Promotion for Guided Bone Regeneration
Provisionally accepted- Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Hospital of Stomatology, Jilin University, Changchun, China
Guided bone regeneration (GBR) technology has been widely used as a reliable method to address alveolar bone defects. However, the existing resorbable guided bone regeneration membranes (GBRMs) are limited to maintain the osteogenic space effectively due to poor mechanical strength and rapid degradation rate, etc. By combining the phase inversion method and electrospinning method, functional grade bilayer membranes (FGBM) with gradient structure and composition of poly(lactic-coglycolic acid) (PLGA), nano-hydroxyapatite (nHA), and gelatin were fabricated in this study. This study comprehensively evaluated the physicochemical and biological properties of the prepared FGBM, encompassing structural and morphological characterization, mechanical properties, in vitro biodegradation, cell behaviors, and in vivo osteogenic bioactivity. The findings demonstrated the successful fabrication of PLGA/nHA/gelatin FGBM with an asymmetric structure, exhibiting enhanced hydrophilic, mechanical, and degradation properties. The inclusion of gelatin not only brought better biological integration, but also enhanced the binding affinity between electrospun fiber layer and phase inversion layer. The fabricated FGBM with a 30% nHA mass fraction and a PLGA/gelatin mass ratio of 1:1 exhibited both good barrier function and the optimal osteogenic bioactivities in vitro and in vivo. Consequently, our findings substantiated the viability of FGBM for guided bone regeneration.
Keywords: Guided bone regeneration, poly(lactic-co-glycolic acid), nanohydroxyapatite, Gelatin, Osteogenesis
Received: 22 Jun 2024; Accepted: 17 Sep 2024.
Copyright: © 2024 Li, Ding, Zhou, Li, Wang, Wang and Fu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Jiaxin Ding, Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Hospital of Stomatology, Jilin University, Changchun, China
Tao Zhou, Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Hospital of Stomatology, Jilin University, Changchun, China
Bolun Li, Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Hospital of Stomatology, Jilin University, Changchun, China
Jingjing Wang, Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Hospital of Stomatology, Jilin University, Changchun, China
Hanchi Wang, Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Hospital of Stomatology, Jilin University, Changchun, China
Li Fu, Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Hospital of Stomatology, Jilin University, Changchun, China
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