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ORIGINAL RESEARCH article

Front. Pharmacol.
Sec. Ethnopharmacology
Volume 15 - 2024 | doi: 10.3389/fphar.2024.1451300
This article is part of the Research Topic Natural Products and Immune Inflammation: Mechanistic Understanding Based on Systems Biology View all 14 articles

Combining single-cell RNA sequencing and network

Provisionally accepted
Danqi Liu Danqi Liu 1,2Chaofeng Wei Chaofeng Wei 1Lu Guan Lu Guan 1Wenhan Ju Wenhan Ju 1Shan Xiang Shan Xiang 1*Fang Lian Fang Lian 3*
  • 1 First College of Clinical Medicine, Shandong University of Traditional Chinese Medicine, Jinan, Shandong Province, China
  • 2 Department of Biomedical and Molecular Sciences, School of Medicine, Faculty of Health Sciences, Queen's University, Kingston, Alberta, Canada
  • 3 Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, China

The final, formatted version of the article will be published soon.

    Background: Polycystic ovary syndrome (PCOS) is a heterogeneous gynecological 12 endocrine disorder linked to immunity. Cangfu Daotan Decoction (CFDT), a classic 13 Chinese medicine prescription, is particularly effective in treating PCOS, specifically 14 in patients with obesity; however, its specific mechanism remains unclear. 15 Methods: Part 1: Peripheral blood mononuclear cells were collected on egg retrieval 16 day from obese and normal-weight patients with PCOS and healthy women undergoing 17 in vitro fertilization (IVF)-embryo transfer. Next, scRNA-seq was performed to screen 18 the key genes of bese patients with PCOS. Part 2: Active ingredients of CFDT and 19 obesity-related PCOS targets were identified based on public databases, and the binding 20 ability between the active ingredients and targets was analyzed. Part 3: This part was a 21 monocentric, randomized controlled trial. The obese women with PCOS were 22 randomized to CFDT (6 packets/day) or placebo, and the healthy women were included 23 in the blank control group (43 cases per group). The clinical manifestations and 24 laboratory outcomes among the three groups were compared.

    Keywords: Polycystic Ovary Syndrome, Immune abnormalities, Obesity, single-cell RNA sequencing, Network Pharmacology, Cangfu Daotan Decoction

    Received: 19 Jun 2024; Accepted: 26 Sep 2024.

    Copyright: © 2024 Liu, Wei, Guan, Ju, Xiang and Lian. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Shan Xiang, First College of Clinical Medicine, Shandong University of Traditional Chinese Medicine, Jinan, Shandong Province, China
    Fang Lian, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.