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ORIGINAL RESEARCH article

Front. Pharmacol.
Sec. Translational Pharmacology
Volume 15 - 2024 | doi: 10.3389/fphar.2024.1446707

Chen's Peiyuan Tang and Premature Ovarian Failure: Unveiling the Mechanisms Through Network Pharmacology

Provisionally accepted
Xiao Yang Xiao Yang 1Yiming Mao Yiming Mao 2Chong Yao Chong Yao 3Ding-ming Song Ding-ming Song 4Yi-bo He Yi-bo He 5*Wei Shen Wei Shen 6*
  • 1 The Third Clinical School of Medicine and Rehabilitation School, Zhejiang Chinese Medicine University, Hangzhou, Jiangsu Province, China
  • 2 Department of Thoracic Surgery, Suzhou Kowloon Hospital, Shanghai Jiao Tong University School of Medicine, Suzhou, Liaoning Province, China
  • 3 Huzhou Central Hospital, Huzhou, China
  • 4 Department of Urology, First Affiliated Hospital of Jinzhou Medical University, Jinzhou, China
  • 5 First Affiliated Hospital, Zhejiang Chinese Medical University, Hangzhou, China
  • 6 Pharmacy Compounding Center, The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), Hangzhou, Jiangsu Province, China

The final, formatted version of the article will be published soon.

    Premature ovarian failure (POF) is a clinical syndrome affecting women under 40, characterized by amenorrhea, elevated follicle-stimulating hormone (FSH) levels, and diminished estrogen levels, leading to significant fertility issues and increased risks of cardiovascular disease, osteoporosis, and Alzheimer's disease. Traditional Chinese Medicine (TCM), specifically Chen's Peiyuan Tang (CSPYT), offers a promising alternative to conventional hormone replacement therapy (HRT), which carries risks such as cancer. CSPYT, a compound herbal formula, has shown potential in enhancing ovarian function and mitigating autophagy in ovarian granulosa cells, crucial for follicle development and maturation. This study explores CSPYT's therapeutic mechanisms in treating POF, focusing on its modulation of autophagy through network pharmacology and transcriptomics-based analysis, predicting potential interactions and pathways. KGN cell line and rat ovarian granulosa cells were used for in vitro experiment. 4-Hydroperoxy cyclophosphamide(4-HC) stimulation was carried out for establishing the POF cell model. Q-PCR, Western Blot, Transmission electron microscopy to detect the results. Key findings indicate that CSPYT inhibits the MAPK signaling pathway, preventing autophagy overexpression and restoring ovarian granulosa cell function, presenting a multifaceted treatment potential for POF.

    Keywords: Traditional Chinese medicine (TCM), Chen's Peiyuan Tang (CSPYT), Autophagy, MAPK signaling pathway, Network Pharmacology

    Received: 10 Jun 2024; Accepted: 15 Nov 2024.

    Copyright: © 2024 Yang, Mao, Yao, Song, He and Shen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Yi-bo He, First Affiliated Hospital, Zhejiang Chinese Medical University, Hangzhou, China
    Wei Shen, Pharmacy Compounding Center, The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), Hangzhou, Jiangsu Province, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.