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ORIGINAL RESEARCH article

Front. Pharmacol.
Sec. Ethnopharmacology
Volume 15 - 2024 | doi: 10.3389/fphar.2024.1446244
This article is part of the Research Topic Multi-omics Technology: Revealing the Pathogenesis of Diseases and the Mechanism of Drug Efficacy of Major Diseases such as Nutritional Metabolism Disorders and Mental Disorders View all 5 articles

Effects of Weizhuan'an on rats with precancerous lesion of gastric cancer based on regulating gastric mucosal microflora and inflammatory factors

Provisionally accepted
Yu-ting LU Yu-ting LU 1Huayi LIU Huayi LIU 2*Jiaju SHANG Jiaju SHANG 2*Yijia MAO Yijia MAO 2*Lingkai MENG Lingkai MENG 2Changbai GAO Changbai GAO 3*
  • 1 Guangdong Second Provincial General Hospital, Integrated Chinese and Western Medicine Postdoctoral Research Station, School of Medicine, Jinan University, Guangzhou, China
  • 2 Tianjin Academy of Traditional Chinese Medicine Affiliated Hospital, Tianjin, China
  • 3 Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, Hebei, China

The final, formatted version of the article will be published soon.

    Observe the intervention of Weizhuan'an Prescription on rats with precancerous lesion of gastric cancer (PLGC) as well as its regulation on gastric mucosal microflora and inflammatory factors, and to explore the pharmacodynamic mechanism of Weizhuan'an Formula. The rats were divided into blank control group (BCG), low, medium and high dose groups of Weizhuan'an Prescription (LDG, MDG, HDG), and natural recovery group (NRG) at random. The rats in traditional Chinese medicine (TCM) were given corresponding doses of Weizhuan'an Formula, while the rats in NRG and BCG with the equivalent distilled water for 12 weeks. After that, gastric mucosa samples of rats were collected to observe the general and pathological changes of gastric mucosa, the changes of gastric mucosal microflora were detected by 16S rDNA amplicon sequencing, and the inflammatory factors were analyzed by cytokine antibody microarray and western blotting. The results suggest that compared with the BCG, the pathology of gastric mucosa and gastric mucosal microflora and inflammatory factors in rats with PLGC have changed significantly, while Weizhuan'an Formula effectively improved them, especially the MDG and HDG (P<0.05). Compared with the NRG, the abundance of probiotics such as Lactobacillus and Veillonella were increased, while the pathogens such as Proteobacteria and Pseudomonas were decreased (P＜0.05, P＜0.01), the relative contents of IL-2, IL-4, IL-13 and MCP-1 in gastric mucosa were decreased (P＜0.05). Moreover, it can up regulate the DNA-binding transcriptional regulator, ABC-type multidrug transport system, and related enzymes, and affect the signal pathways such as Viral protein interaction with cytokine and cytokine receptor, T cell receptor signaling pathway significantly (P＜0.05, P＜0.01), which can promote drug absorption and utilization and repair damaged gastric mucosa. The study confirmed that Weizhuan'an Prescription can treat rats with PLGC by regulating gastric mucosal microflora and inflammatory factors.

    Keywords: Precancerous lesion of gastric cancer, Gastric Mucosa, Traditional Chinese Medicine, gastric mucosal microflora, Inflammatory factors, Weizhuan'an Prescription

    Received: 09 Jun 2024; Accepted: 24 Jul 2024.

    Copyright: © 2024 LU, LIU, SHANG, MAO, MENG and GAO. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Huayi LIU, Tianjin Academy of Traditional Chinese Medicine Affiliated Hospital, Tianjin, 300120, China
    Jiaju SHANG, Tianjin Academy of Traditional Chinese Medicine Affiliated Hospital, Tianjin, 300120, China
    Yijia MAO, Tianjin Academy of Traditional Chinese Medicine Affiliated Hospital, Tianjin, 300120, China
    Changbai GAO, Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, Hebei, China

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