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ORIGINAL RESEARCH article

Front. Pharmacol.
Sec. Experimental Pharmacology and Drug Discovery
Volume 15 - 2024 | doi: 10.3389/fphar.2024.1429960
This article is part of the Research Topic Reviews in Acute Stroke and Interventional Therapies 2023 View all 5 articles

Investigation of the effects of Periplaneta americana (L.) extract on ischemic stroke based on combined multi-omics of gut microbiota

Provisionally accepted
Xin Yang Xin Yang Canhui Hong Canhui Hong *Tangfei Guan Tangfei Guan *ChengGui Zhang ChengGui Zhang *Pei Yun Xiao Pei Yun Xiao Yongshou Yang Yongshou Yang Huai Xiao Huai Xiao Zhengchun He Zhengchun He *
  • Dali University, Dali, China

The final, formatted version of the article will be published soon.

    Ischemic stroke (IS) is a highly lethal type of cardiovascular and cerebrovascular disease.Improving survival rates and promoting recovery in patients with IS pose significant challenges, however, recent research has identified the gut-brain axis as a therapeutic target.In this study, we evaluated the regulatory effect of Periplaneta americana (L.) extract (PAS840), which has established anti-inflammatory, antioxidant, and neuroprotective effects, on the gut microbiota using a rat model of temporary middle cerebral artery occlusion (tMCAO). We evaluated the protective effects of PAS840 on brain damage in IS rats through TTC (triphenyltetrazolium chloride), Nissl staining, and pathological section analysis.Additionally, we investigated the impact of PAS840 on the gut microbiota and metabolites using 16S rRNA sequencing, untargeted metabolomics of gut contents, and transcriptomics analyses of brain tissues to explore its mechanism of action. PAS840 intervention resulted in significant changes in the gut microbiota, including an increase in the abundance of probiotic flora, decrease in the abundance of harmful flora, and significant changes in metabolite profiles. It also attenuated brain damage, decreased platelet activity, inhibited oxidative stress and genes related to inflammation, and improved neurological function in rats. These findings suggest that PAS840 has preventive and therapeutic effects against IS via the gut-brain axis by regulating the gut microbiota and related metabolites. Accordingly, PAS840 is a candidate therapeutic drug for further research.

    Keywords: Periplaneta americana (L.), ischemic stroke, gut-brain axis, Gut Microbiota, Metabolomics, Transcriptomics

    Received: 09 May 2024; Accepted: 13 Nov 2024.

    Copyright: © 2024 Yang, Hong, Guan, Zhang, Xiao, Yang, Xiao and He. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Canhui Hong, Dali University, Dali, China
    Tangfei Guan, Dali University, Dali, China
    ChengGui Zhang, Dali University, Dali, China
    Zhengchun He, Dali University, Dali, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.