Skip to main content

ORIGINAL RESEARCH article

Front. Pharmacol.
Sec. Ethnopharmacology
Volume 15 - 2024 | doi: 10.3389/fphar.2024.1427355
This article is part of the Research Topic Central Asian Medicinal Plants and Fungi as Sources of Biologically Active Extracts and Metabolites View all 5 articles

Pomegranate flower polysaccharide improves mastitis in mice by regulating intestinal flora and restoring the blood-milk barrier

Provisionally accepted
Jianlong Li Jianlong Li 1Wen Yin Wen Yin 2*Yuan Liang Yuan Liang 1*Zhaoran Yang Zhaoran Yang 1*Liangliang Li Liangliang Li 3*Yi Wu Yi Wu 2*Yabin Lu Yabin Lu 1Zhanhai Mai Zhanhai Mai 1Xingjian Yu Xingjian Yu 4Zhenping Zhang Zhenping Zhang 1*Saifuding Abula Saifuding Abula 1*Adelijiang Wusiman Adelijiang Wusiman 1*Qingyong Guo Qingyong Guo 1*
  • 1 Xinjiang Agricultural University, Ürümqi, Xinjiang Uyghur Region, China
  • 2 Nanjing Agricultural University, Nanjing, China
  • 3 Henan Agricultural University, Zhengzhou, Henan Province, China
  • 4 University of California, Davis, Davis, California, United States

The final, formatted version of the article will be published soon.

    This study explored the inhibitory effect of pomegranate flower polysaccharide (PFPS) on mastitis through in vitro and in vivo models. PFPS is a new type of polysaccharide isolated and extracted from pomegranate flowers. The result revealed that PFPS consists of GalA, Ara, and Gal, and the residues consist of 1,4-GalpA, 1,4-Galp, and 1,3,6-Galp, which contain HG-type and RG-I-type pectin structural domains. In vitro studies showed that PFPS could inhibit LPS-enhanced phagocytosis of RAW 264.7 cells and the release of IL-1β, IL-10, and TNF-α. In vivo, studies showed that PFPS improved xylene-induced mouse ear swelling and carrageenan-induced mouse paw edema by inhibiting inflammatory factors. In the mouse mastitis model, PFPS significantly improved LPS-induced inflammation and oxidative stress in mammary tissue. Intestinal flora sequencing results showed that PFPS could effectively regulate the intestinal flora of mice, reduce the relative abundance of pathogenic bacteria Oscillospira and AF12, and increase the probiotics Blautia, Parabacteroides, Allobaculum, and Clostridiaceae_Clostridium. Therefore ， PFPS ultimately played a role in preventing mastitis by regulating the intestinal flora and further improving the blood-milk barrier. This study provides a scientific basis for PFPS as a potential candidate drug for the treatment of mastitis.

    Keywords: Punica granatum, anti-inflammation effect, Gut Microbiota, RAW 264.7, Ear swelling, Paw swelling

    Received: 03 May 2024; Accepted: 22 Jul 2024.

    Copyright: © 2024 Li, Yin, Liang, Yang, Li, Wu, Lu, Mai, Yu, Zhang, Abula, Wusiman and Guo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Wen Yin, Nanjing Agricultural University, Nanjing, China
    Yuan Liang, Xinjiang Agricultural University, Ürümqi, 830052, Xinjiang Uyghur Region, China
    Zhaoran Yang, Xinjiang Agricultural University, Ürümqi, 830052, Xinjiang Uyghur Region, China
    Liangliang Li, Henan Agricultural University, Zhengzhou, 450002, Henan Province, China
    Yi Wu, Nanjing Agricultural University, Nanjing, China
    Zhenping Zhang, Xinjiang Agricultural University, Ürümqi, 830052, Xinjiang Uyghur Region, China
    Saifuding Abula, Xinjiang Agricultural University, Ürümqi, 830052, Xinjiang Uyghur Region, China
    Adelijiang Wusiman, Xinjiang Agricultural University, Ürümqi, 830052, Xinjiang Uyghur Region, China
    Qingyong Guo, Xinjiang Agricultural University, Ürümqi, 830052, Xinjiang Uyghur Region, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.