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ORIGINAL RESEARCH article
Front. Pharmacol.
Sec. Ethnopharmacology
Volume 15 - 2024 |
doi: 10.3389/fphar.2024.1420411
Danggui Buxue decoction regulates the immune function and intestinal microbiota of cyclophosphamide induced immunosuppressed mice
Provisionally accepted
Huan Huang
1
Yufei Xie
1
Xifeng Li
1
Fuxing Gui
1
Pingrui Yang
1
Yutao Li
2
Li Zhang
3
Hongxu Du
1
Shicheng Bi
1
Liting CAO
1*- 1 Southwest University, Chongqing, China
- 2 Weifang Academy of Agricultural Sciences, Weifang, Shandong Province, China
- 3 HanZhong Center for Disease Control and Prevention, Hanzhong, China
This study was conducted to investigate the protective effects of Danggui Buxue decoction (DBD) on the immunity, intestinal mucosal barrier function and gut microbiota in cyclophosphamide (CY) induced immunosuppressed (IS) mice. This study takes mice as the research object. A mouse model of IS induced by CY.Pathological examination, ELISA and molecular biology techniques were used to investigate the immunomodulatory effects of DBD. The results showed that DBD alleviated spleen tissue damage and restored impaired immune functions, such as increased thymus index and CD4+/CD8+ subsets of spleen lymphocytes (P < 0.05). In addition, DBD could increase ileum villi length and the ratio of villi length to crypt depth (V/C), and decrease crypt depth (P < 0.05). Moreover, DBD administration upregulated the expression of ZO-1, Occludin, Claudin-1, MUC-2 mRNA in ileum (P < 0.05). And the secretions of sIgA and ZO-1 in ileum were also significantly improved (P < 0.05). Furthermore, the administration of DBD can increase the diversity of gut microbiota, improve the composition of intestinal flora and increase the relative abundance of beneficial genus, such as Bacteroides. In conclusion, our study indicates that DBD has potential application for enhancing immunity.
Keywords: Danggui buxue decoction, Immunosuppression, intestinal mucosal immunity, Gut Microbiota, Immune function
Received: 20 Apr 2024; Accepted: 30 Jul 2024.
Copyright: © 2024 Huang, Xie, Li, Gui, Yang, Li, Zhang, Du, Bi and CAO. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Liting CAO, Southwest University, Chongqing, China
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