Skip to main content

ORIGINAL RESEARCH article

Front. Pharmacol.
Sec. Ethnopharmacology
Volume 15 - 2024 | doi: 10.3389/fphar.2024.1410419

Clinacanthus nutans extract lowers periodontal inflammation under high glucose conditions via inhibiting NF-κB signaling pathway

Provisionally accepted
Aussara Panya Aussara Panya 1,2,3*Saruda Thongyim Saruda Thongyim 1,2Thomas A. Wright Thomas A. Wright 4Thida Kaewkod Thida Kaewkod 1,2George S. Baillie George S. Baillie 4Yingmanee Tragoolpua Yingmanee Tragoolpua 1,2Siriphorn Jangsutthivorawat Siriphorn Jangsutthivorawat 2
  • 1 Chiang Mai University, Chiang Mai, Thailand
  • 2 Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand
  • 3 Faculty of Science, Chiang Mai University, Chiang Mai, Thailand
  • 4 School of Cardiovascular and Metabolic Health, University of Glasgow, Glasgow, United Kingdom

The final, formatted version of the article will be published soon.

    Periodontal disease is more prevalent in patients with diabetes, and it has a negative impact on their quality of life. Inhibiting the infection and inflammation processes that cause periodontal disease can reduce the severity of the disease and chances of serious complications. In this study, we aimed to demonstrate the effectiveness of Clinacanthus nutans extract in reducing the inflammation in gingival fibroblast cells induced by Porphyromonas gingivalis lipopolysaccharide (LPS). Stimulation with LPS under high glucose conditions led to increased inflammation compared to low glucose conditions. Treatment of C. nutans extract significantly reduced the expression of these pro-inflammatory cytokines and chemokines. At a concentration of 50 µg/mL, it reduced the relative expression of IL6, IL8, and CXCL10 to 0.51 ± 0.09, 0.6 ± 0.19, and 0.09 ± 0.02, respectively, compared to the non-treatment control, accompanied by a decrease in secreted protein as measured by ELISA. Additionally, application of C. nutans extract markedly suppressed the NF-κB signaling pathway by reducing the phosphorylated form of IκBα, NF-κB p65, and nuclear translocation of NF-κB, along with a decrease in COX2, a key mediator in the inflammatory pathway. Furthermore, analysis of RNA sequencing data indicated that the extract clearly reversed the gene expression changes induced by LPS. This was particularly true for the signaling mediators and inflammatory genes in response to NF-κB, JAK/STAT, and TNF signaling pathways. Our finding highlights the potential of C. nutans extract to alleviate inflammation and suggest its potential as a treatment for periodontal disease in patients with diabetes.

    Keywords: Periodontal disease, Inflammation, Clinacanthus nutans, Herb extract, NF-κB signaling pathway 1. Introduction Periodontal disease is a chronic inflammatory disease caused by bacterial infections

    Received: 02 May 2024; Accepted: 29 Jul 2024.

    Copyright: © 2024 Panya, Thongyim, Wright, Kaewkod, Baillie, Tragoolpua and Jangsutthivorawat. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Aussara Panya, Chiang Mai University, Chiang Mai, Thailand

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.