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ORIGINAL RESEARCH article

Front. Pharmacol.
Sec. Drug Metabolism and Transport
Volume 15 - 2024 | doi: 10.3389/fphar.2024.1401275

Cyadox, a quinoxaline-1,4-dioxide derivative: transport, tissue distribution and depletion, and hepatotoxicity studies

Provisionally accepted
Zhu Tao Zhu Tao 1Changchun Li Changchun Li 1Aiqun Zhang Aiqun Zhang 1Zhilin Zhang Zhilin Zhang 1Jing Huang Jing Huang 1Sechenchogt Harnud Sechenchogt Harnud 1,2*
  • 1 Hubei Engineering University, Xiaogan, Hubei, China
  • 2 College of Life Science and Technology, Hubei Engineering University, Xiaogan, China

The final, formatted version of the article will be published soon.

    Background: Cyadox (CYA) is a derivative of quinoxaline 1,4-dioxide and a safe and effective synthetic antibacterial agent.Objective: This study aimed to explore the drug transport in blood, distribution, depletion and hepatotoxicity of drugs in animals.The transport of CYA in blood was studied using fluorescence, circular dichroism (CD) and molecular docking methods. Tissue distribution and depletion of CYA in rats were evaluated following oral administration of [3H]-CYA at different doses. Hepatotoxicity of drugs evaluated by transcriptomics.Results: During transport in the bloodstream, the drug binds to bovine serum albumin (BSA) by hydrogen bonding and has only one binding site. Hydrogen bonds were formed between O (2) of CYA and ARG208, O (3) of CYA and LEU480, VAL481. The secondary protein conformation of BSA changed after binding with an increase in α-helix and a decrease in β-strand. After a single oral administration of [ 3 H]-CYA, it was excreted rapidly within 7 days, with 34.81% from the urine and 60.25% from the feces. Higher and sustained levels of radioactivity were detected in the liver during the post-dose period, suggesting that the drug may concentrate in the liver. The transcriptomic data indicates that CYA exhibits low hepatotoxicity. However, there are indications that it may have an impact on steroid biosynthesis.This study could serve as a basis for conducting further studies on the use of CYA in food animals and improving the pharmacologic, pharmacokinetic, and toxicologic effects of CYA on food animals.

    Keywords: Cyadox, distribution, Docking, Radioisotopic tracing, Hepatotoxicity

    Received: 15 Mar 2024; Accepted: 06 Sep 2024.

    Copyright: © 2024 Tao, Li, Zhang, Zhang, Huang and Harnud. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Sechenchogt Harnud, College of Life Science and Technology, Hubei Engineering University, Xiaogan, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.