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ORIGINAL RESEARCH article

Front. Pharmacol.
Sec. Pharmacology of Anti-Cancer Drugs
Volume 15 - 2024 | doi: 10.3389/fphar.2024.1400186
This article is part of the Research Topic Regulated cell death (RCD) in cancer: signaling pathways activated by natural products and their nano-formulations View all 4 articles

Transcriptome Analysis Revealed the Genes and Major Pathways Involved in Prunetrin Treated Hepatocellular Carcinoma

Provisionally accepted
ABUYASEER ABUSALIYA ABUYASEER ABUSALIYA 1Hun H. Kim Hun H. Kim 1Preethi Vetrivel Preethi Vetrivel 1Pritam B. Bhosale Pritam B. Bhosale 1Se H. Jeong Se H. Jeong 1Min Y. Park Min Y. Park 1Si J. Lee Si J. Lee 2Gon S. Kim Gon S. Kim 1*
  • 1 Research Institute of Life science and College of Veterinary Medicine, Gyeongsang National University, Jinju, South Gyeongsang, Republic of Korea
  • 2 Daegu-Gyeongbuk Medical Innovation Foundation (DGMIF), Daegu, Daegu, Republic of Korea

The final, formatted version of the article will be published soon.

    Liver cancer represents a complex and severe ailment that poses tough challenges to global healthcare. Transcriptome sequencing plays a crucial role in enhancing our understanding of cancer biology and accelerating the development of more effective methods for cancer diagnosis and treatment. In the course of our current investigation, we identified a total of 1149 differentially expressed genes (DEGs), encompassing 499 upregulated and 650 downregulated genes, subsequent to prunetrin (PUR) treatment. Our methodology encompassed gene and pathway enrichment analysis, functional annotation, KEGG pathway assessments, and protein-protein interaction (PPI) analysis of the DEGs. The preeminent genes within the DEGs were found to be associated with apoptotic processes, cell cycle regulation, the PI3k/Akt pathway, the MAPK pathway, and the mTOR pathway. Furthermore, key apoptotic-related genes exhibited close interconnections and cluster analysis found three interacting hub genes namely TP53, TGFB1 and CASP8. Validation of these genes was achieved through GEPIA and western blotting. Collectively, our findings provide insights into the functional landscape of liver cancer-related genes, shedding light on the molecular mechanisms driving disease progression and highlighting potential targets for therapeutic intervention. Deleted: Transcriptome sequencing assumes a pivotal role in 40 augmenting comprehension of cancer biology and expediting the 41 formulation of more efficacious modalities for cancer diagnosis and

    Keywords: Hepatocellular Carcinoma, Hep3B cells, Transcriptome sequencing, RNA-Seq, Prunetrin

    Received: 13 Mar 2024; Accepted: 14 Oct 2024.

    Copyright: © 2024 ABUSALIYA, Kim, Vetrivel, Bhosale, Jeong, Park, Lee and Kim. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Gon S. Kim, Research Institute of Life science and College of Veterinary Medicine, Gyeongsang National University, Jinju, South Gyeongsang, Republic of Korea

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.